ArticleBMC research notes2010
A new mutant genetic resource for tomato crop improvement by TILLING technology.
Article in BMC research notes, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed.
- Arbuscular mycorrhizal symbiosis in tomato roots with a diverse range of carotene accumulation.Mycorrhiza · 2026Article
- EMS-induced genomic variation and QTL-seq analysis of safflower spininess through whole genome sequencing (WGS).BMC genomics · 2025Article
- What metabolomics has taught us about tomato fruit ripening and quality.Journal of experimental botany · 2025Review
- Can genome editing help transitioning to agroecology?iScience · 2024Review
- The tomato calcium-permeable channel 4.1 (SlOSCA4.1) is a susceptibility factor for pepino mosaic virus.Plant biotechnology journal · 2023Article
- Article
- Characterization of prolycopene-accumulated TanJournal of biosciences · 2023Article
- Augmenting tomato functional genomics with a genome-wide induced genetic variation resource.Frontiers in plant science · 2023Article
- Is it the end of TILLING era in plant science?Frontiers in plant science · 2023Review
- Targeted Identification of Rice Grain-Associated Gene Allelic Variation Through Mutation Induction, Targeted Sequencing, and Whole Genome Sequencing Combined with a Mixed-Samples Strategy.Rice (New York, N.Y.) · 2022Article
- Natural and artificial sources of genetic variation used in crop breeding: A baseline comparator for genome editing.Frontiers in genome editing · 2022Review
- Current trends and insights on EMS mutagenesis application to studies on plant abiotic stress tolerance and development.Frontiers in plant science · 2022Review
- Discovery and Characterization of a Novel TomatoGenes · 2021Article
- The Global Dimension of Tomato Yellow Leaf Curl Disease: Current Status and Breeding Perspectives.Microorganisms · 2021Review
- Targeted editing of tomato carotenoid isomerase reveals the role of 5' UTR region in gene expression regulation.Plant cell reports · 2021Article
- Application of Genome Editing in Tomato Breeding: Mechanisms, Advances, and Prospects.International journal of molecular sciences · 2021Review
- Applied mutagenesis could improve economically important traits in bitter gourd (Journal of genetics · 2021Article
- Applications of Multi-Omics Technologies for Crop Improvement.Frontiers in plant science · 2021Review
- Unraveling Cuticle Formation, Structure, and Properties by Using Tomato Genetic Diversity.Frontiers in plant science · 2021Review
- Highly Efficient and Comprehensive Identification of Ethyl Methanesulfonate-Induced Mutations inFrontiers in plant science · 2021Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the last decade, the availability of gene sequences of many plant species, including tomato, has encouraged the development of strategies that do not rely on genetic transformation techniques (GMOs) for imparting desired traits in crops. One of these new emerging technology is TILLING (Targeting Induced Local Lesions In Genomes), a reverse genetics tool, which is proving to be very valuable in creating new traits in different crop species.
resultsTo apply TILLING to tomato, a new mutant collection was generated in the genetic background of the processing tomato cultivar Red Setter by treating seeds with two different ethylemethane sulfonate doses (0.7% and 1%). An associated phenotype database, LycoTILL, was developed and a TILLING platform was also established. The interactive and evolving database is available online to the community for phenotypic alteration inquiries. To validate the Red Setter TILLING platform, induced point mutations were searched in 7 tomato genes with the mismatch-specific ENDO1 nuclease. In total 9.5 kb of tomato genome were screened and 66 nucleotide substitutions were identified. The overall mutation density was estimated and it resulted to be 1/322 kb and 1/574 kb for the 1% EMS and 0.7% EMS treatment respectively.
conclusionsThe mutation density estimated in our collection and its comparison with other TILLING populations demonstrate that the Red Setter genetic resource is suitable for use in high-throughput mutation discovery. The Red Setter TILLING platform is open to the research community and is publicly available via web for requesting mutation screening services.
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