Evidence map›Paper›PMID 20222986›Full record

ArticleProteome science2010

Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins.

Lijun Zhang, Xiaofang Jia, Xiaojun Zhang, Jianjun Sun, Xia Peng, Tangkai Qi, Fang Ma, Lin Yin, Yamin Yao, Chao Qiu and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proteome science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 43 citations in OpenAlex.

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  11. Plasma membrane proteomic analysis of human osteosarcoma and osteoblastic cells: revealing NDRG1 as a marker for osteosarcoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2011
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Lijun Zhang *Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Xiaofang Jia *Shanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Xiaojun Zhang *Neurosurgery, Fuzhou General Hospital, Fuzhou 350025, PR China.
Jianjun SunShanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Xia PengShanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Tangkai QiShanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Fang MaShanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Lin YinShanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Yamin YaoShanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Chao QiuInstitutes of Biomedical Sciences, Fudan University, Shanghai, 200032, PR China.
Hongzhou LuShanghai Public Health Clinical Center affiliated to Fudan University, Shanghai, 201508, PR China.
Fudan University · CNShanghai Public Health Clinical Center · CNFuzhou General Hospital of Nanjing Military Command · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe human immunodeficiency virus type 1 (HIV-1) pandemic has continued unabated for nearly 30 years. To better understand the influence of virus on host cells, we performed the differential proteome research of peripheral blood mononuclear cells (PBMCs) from HIV-positive patients and healthy controls.

results26 protein spots with more than 1.5-fold difference were detected in two dimensional electrophoresis (2DE) gels. 12 unique up-regulated and one down-regulated proteins were identified in HIV-positive patients compared with healthy donors. The mRNA expression of 10 genes was analyzed by real time RT-PCR. It shows that the mRNA expression of talin-1, vinculin and coronin-1C were up-regulated in HIV positive patients and consistent with protein expression. Western blotting analysis confirmed the induction of fragments of vinculin, talin-1 and filamin-A in pooled and most part of individual HIV-positive clinical samples. Bioinformatic analysis showed that a wide host protein network was disrupted in HIV-positive patients.

conclusionsTogether, this work provided useful information to facilitate further investigation of the underlying mechanism of HIV and host cell protein interactions, and discovered novel potential biomarkers such as fragment of vinculin, filamin-A and talin-1 for anti-HIV research.

Identifiers

PMID20222986
PMCPMC2850332
OpenAlexW2044825233

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.