ArticleExtremophiles : life under extreme conditions2010
Inducible and constitutive promoters for genetic systems in Sulfolobus acidocaldarius.
Article in Extremophiles : life under extreme conditions, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 77 citations in OpenAlex.
- Sugar-inducible promoters for manipulation of core metabolic pathways in the thermophilic acetogenApplied and environmental microbiology · 2025Article
- Establishment of the CRISPR-Cpf1 gene editing system inSynthetic and systems biotechnology · 2025Article
- 5'-untranslated region sequences enhance plasmid-based protein production inFrontiers in microbiology · 2024Article
- Archaea as a Model System for Molecular Biology and Biotechnology.Biomolecules · 2023Review
- Progress and Challenges in Archaeal Genetic Manipulation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Characterization of a Novel Thermostable Dye-Linked l-Lactate Dehydrogenase Complex and Its Application in Electrochemical Detection.International journal of molecular sciences · 2021Article
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- Genome Editing of the Anaerobic Thermophile Thermoanaerobacter ethanolicus Using Thermostable Cas9.Applied and environmental microbiology · 2020Article
- CRISPR-mediated gene silencing reveals involvement of the archaeal S-layer in cell division and virus infection.Nature communications · 2019Article
- Biotechnology of extremely thermophilic archaea.FEMS microbiology reviews · 2018Review
- A regulatory RNA is involved in RNA duplex formation and biofilm regulation in Sulfolobus acidocaldarius.Nucleic acids research · 2018Article
- A conserved hexanucleotide motif is important in UV-inducible promoters in Sulfolobus acidocaldarius.Microbiology (Reading, England) · 2017Article
- Guide-independent DNA cleavage by archaeal Argonaute from Methanocaldococcus jannaschii.Nature microbiology · 2017Article
- Expanding the archaellum regulatory network - the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius.MicrobiologyOpen · 2017Article
- Review
- Disruption of the gene encoding restriction endonuclease SuaI and development of a host-vector system for the thermoacidophilic archaeon Sulfolobus acidocaldarius.Extremophiles : life under extreme conditions · 2016Article
- The archaeal Ced system imports DNA.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Involvement of a eukaryotic-like ubiquitin-related modifier in the proteasome pathway of the archaeon Sulfolobus acidocaldarius.Nature communications · 2015Article
- DNA Processing Proteins Involved in the UV-Induced Stress Response of Sulfolobales.Journal of bacteriology · 2015Article
- Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals.Frontiers in microbiology · 2015Review
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Central to genetic work in any organism are the availability of a range of inducible and constitutive promoters. In this work we studied several promoters for use in the hyperthermophilic archaeon Sulfolobus acidocaldarius. The promoters were tested with the aid of an E. coli-Sulfolobus shuttle vector in reporter gene experiments. As the most suitable inducible promoter a maltose inducible promoter was identified. It comprises 266 bp of the sequence upstream of the gene coding for the maltose/maltotriose binding protein (mbp, Saci_1165). Induction is feasible with either maltose or dextrin at concentrations of 0.2-0.4%. The highest increase in expression (up to 17-fold) was observed in late exponential and stationary phase around 30-50 h after addition of dextrin. Whereas in the presence of glucose and xylose higher basal activity and reduced inducibility with maltose is observed, sucrose can be used in the growth medium additionally without affecting the basal activity or the inducibility. The minimal promoter region necessary could be narrowed down to 169 bp of the upstream sequence. The ABCE1 protein from S. solfataricus was successfully expressed under control of the inducible promoter with the shuttle vector pC and purified from the S. acidocaldarius culture with a yield of about 1 mg L(-1) culture. In addition we also determined the promoter strength of several constitutive promoters.
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