ArticlePLoS genetics2010
PPS, a large multidomain protein, functions with sex-lethal to regulate alternative splicing in Drosophila.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 31 citations in OpenAlex.
- Sex-specific metabolic regulation by the Drosophila RNA-binding protein Nab2.bioRxiv : the preprint server for biology · 2026Article
- Domain acquisition enabled functional expansion of the TFIIS transcription factor family.Cell & bioscience · 2025Review
- SPOC domain proteins in health and disease.Genes & development · 2023Review
- The Dengue Virus Nonstructural Protein 1 (NS1) Interacts with the Putative Epigenetic Regulator DIDO1 to Promote Flavivirus Replication in Mosquito Cells.Journal of virology · 2022Article
- Blood biomarkers representing maternal-fetal interface tissues used to predict early-and late-onset preeclampsia but not COVID-19 infection.Computational and structural biotechnology journal · 2022Article
- Role of Hakai in mNature communications · 2021Article
- Gtsf1 is essential for proper female sex determination and transposon silencing in the silkworm, Bombyx mori.PLoS genetics · 2020Article
- Structure of the BRK domain of the SWI/SNF chromatin remodeling complex subunit BRG1 reveals a potential role in protein-protein interactions.Protein science : a publication of the Protein Society · 2020Article
- Dido3-dependent SFPQ recruitment maintains efficiency in mammalian alternative splicing.Nucleic acids research · 2019Article
- A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling.Developmental cell · 2018Article
- Xio is a component of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- A Unique pH-Dependent Recognition of Methylated Histone H3K4 by PPS and DIDO.Structure (London, England : 1993) · 2017Article
- Promiscuity in post-transcriptional control of gene expression: Drosophila sex-lethal and its regulatory partnerships.FEBS letters · 2017Review
- DIDO as a Switchboard that Regulates Self-Renewal and Differentiation in Embryonic Stem Cells.Stem cell reports · 2017Article
- Bombyx mori P-element Somatic Inhibitor (BmPSI) Is a Key Auxiliary Factor for Silkworm Male Sex Determination.PLoS genetics · 2017Article
- The autoregulatory loop: A common mechanism of regulation of key sex determining genes in insects.Journal of biosciences · 2016Review
- spenito is required for sex determination in Drosophila melanogaster.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Screen for mitochondrial DNA copy number maintenance genes reveals essential role for ATP synthase.Molecular systems biology · 2014Article
- Dido3 PHD modulates cell differentiation and division.Cell reports · 2013Article
- Sex-lethal enables germline stem cell differentiation by down-regulating Nanos protein levels during Drosophila oogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Alternative splicing controls the expression of many genes, including the Drosophila sex determination gene Sex-lethal (Sxl). Sxl expression is controlled via a negative regulatory mechanism where inclusion of the translation-terminating male exon is blocked in females. Previous studies have shown that the mechanism leading to exon skipping is autoregulatory and requires the SXL protein to antagonize exon inclusion by interacting with core spliceosomal proteins, including the U1 snRNP protein Sans-fille (SNF). In studies begun by screening for proteins that interact with SNF, we identified PPS, a previously uncharacterized protein, as a novel component of the machinery required for Sxl male exon skipping. PPS encodes a large protein with four signature motifs, PHD, BRK, TFS2M, and SPOC, typically found in proteins involved in transcription. We demonstrate that PPS has a direct role in Sxl male exon skipping by showing first that loss of function mutations have phenotypes indicative of Sxl misregulation and second that the PPS protein forms a complex with SXL and the unspliced Sxl RNA. In addition, we mapped the recruitment of PPS, SXL, and SNF along the Sxl gene using chromatin immunoprecipitation (ChIP), which revealed that, like many other splicing factors, these proteins bind their RNA targets while in close proximity to the DNA. Interestingly, while SNF and SXL are specifically recruited to their predicted binding sites, PPS has a distinct pattern of accumulation along the Sxl gene, associating with a region that includes, but is not limited to, the SxlPm promoter. Together, these data indicate that PPS is different from other splicing factors involved in male-exon skipping and suggest, for the first time, a functional link between transcription and SXL-mediated alternative splicing. Loss of zygotic PPS function, however, is lethal to both sexes, indicating that its role may be of broad significance.
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