ArticleJournal of clinical microbiology2010
Identification and genotyping of Mycobacterium tuberculosis complex species by use of a SNaPshot Minisequencing-based assay.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Mycobacterium tuberculosis mixed infections and drug resistance in sub-Saharan Africa: a systematic review.African health sciences · 2022Pooled it
- Sugar transporter SLC45 gene family members: roles in malignant tumors and research progress.Molecular biology reports · 2026Review
- Real-time PCR assay for robust detection and global surveillance of the Mycobacterium tuberculosis Haarlem genotype.Scientific reports · 2025Article
- Evaluation of PCRAfrican health sciences · 2021Article
- Molecular method for the semiquantitative identification of gastrointestinal nematodes in domestic ruminants.Parasitology research · 2020Article
- Species-Specific Interactions of Arr with RplK Mediate Stringent Response in Bacteria.Journal of bacteriology · 2018Article
- Clinical implication of novel drug resistance-conferring mutations in resistant tuberculosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Review
- Optimizing and accelerating the assignation of lineages in Mycobacterium tuberculosis using novel alternative single-tube assays.PloS one · 2017Article
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- Efficient SNP Discovery by Combining Microarray and Lab-on-a-Chip Data for Animal Breeding and Selection.Microarrays (Basel, Switzerland) · 2015Review
- Molecular Epidemiology of Mycoplasma pneumoniae: Genotyping Using Single Nucleotide Polymorphisms and SNaPshot Technology.Journal of clinical microbiology · 2015Article
- An efficient alternative marker for specific identification of Mycobacterium tuberculosis.World journal of microbiology & biotechnology · 2014Article
- Novel DNA chip based on a modified DigiTag2 assay for high-throughput species identification and genotyping of Mycobacterium tuberculosis complex isolates.Journal of clinical microbiology · 2014Article
- Single nucleotide polymorphism (SNP) analysis used for the phylogeny of the Mycobacterium tuberculosis complex based on a pyrosequencing assay.BMC microbiology · 2014Article
- Tuberculosis epidemiology and selection in an autochthonous Siberian population from the 16th-19th century.PloS one · 2014Article
- inTB - a data integration platform for molecular and clinical epidemiological analysis of tuberculosis.BMC bioinformatics · 2013Article
- Three multiplex snapshot assays for SNP genotyping in candidate innate immune genes.BMC research notes · 2013Article
- Single nucleotide polymorphisms in Mycobacterium tuberculosis and the need for a curated database.Tuberculosis (Edinburgh, Scotland) · 2013Review
- Two new rapid SNP-typing methods for classifying Mycobacterium tuberculosis complex into the main phylogenetic lineages.PloS one · 2012Article
- Comparison of an automated repetitive sequence-based PCR microbial typing system with IS6110-restriction fragment length polymorphism for epidemiologic investigation of clinical Mycobacterium tuberculosis isolates in Korea.The Korean journal of laboratory medicine · 2011Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to investigate the use of the SNaPshot minisequencing method for the identification of Mycobacterium tuberculosis complex (MTBC) isolates to the species level and for further genotyping of M. tuberculosis isolates. We developed an innovative strategy based on two multiplex allele-specific minisequencing assays that allowed detection of eight species-specific and eight lineage-specific single nucleotide polymorphisms (SNPs). Each assay consisted of an eightplex PCR amplification, followed by an eightplex minisequencing reaction with the SNaPshot multiplex kit (Applied Biosystems) and, finally, analysis of the extension products by capillary electrophoresis. The whole strategy was developed with a panel of 56 MTBC strains and 15 negative controls. All MTBC strains tested except one M. africanum clinical isolate were accurately identified to the species level, and all M. tuberculosis isolates were successfully further genotyped. This two-step strategy based on SNaPshot minisequencing allows the simultaneous differentiation of closely related members of the MTBC, the distinction between principal genetic groups, and the characterization of M. tuberculosis isolates into one of the seven prominent SNP cluster groups (SCGs) and could be a useful tool for diagnostic and epidemiological purposes.
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