ArticleJournal of clinical microbiology2010
New polymorphic microsatellite markers able to distinguish among Candida parapsilosis sensu stricto isolates.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 89 citations in OpenAlex.
- Candida parapsilosis is a significant neonatal pathogen: a systematic review and meta-analysis.The Pediatric infectious disease journal · 2013Pooled it
- Comparative genotypic analysis of Candida krusei and Candida parapsilosis isolates from human and animal clinical samples through MLVA and RAPD-PCR profiling.GMS hygiene and infection control · 2026Article
- Biofilm-related characteristics ofFrontiers in cellular and infection microbiology · 2026Article
- Silent Persistence: Molecular Evidence of Clonal Transmission in Fluconazole-ResistantJournal of fungi (Basel, Switzerland) · 2025Article
- Clinical impact of fluconazole-resistantFuture microbiology · 2025Review
- Emerging fluconazole-resistantFrontiers in microbiology · 2025Article
- Phenotypic and genomic characterization of azole resistance in PortugueseFrontiers in fungal biology · 2025Article
- Short Tandem Repeat Genotyping of Medically Important Fungi: A Comprehensive Review of a Powerful Tool with Extensive Future Potential.Mycopathologia · 2024Review
- A cluster ofAntimicrobial stewardship & healthcare epidemiology : ASHE · 2024Article
- Article
- Antifungal Resistance and Genotyping of ClinicalJournal of fungi (Basel, Switzerland) · 2023Article
- The first established microsatellite markers to distinguishJournal of clinical microbiology · 2023Article
- The Gastrointestinal Tract Is Pinpointed as a Reservoir ofJournal of fungi (Basel, Switzerland) · 2023Article
- Improving the Detection of Epidemic Clones inMicroorganisms · 2023Article
- Genotypic Diversity ofJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- Evolution of Fluconazole Resistance Mechanisms and Clonal Types ofAntimicrobial agents and chemotherapy · 2022Article
- Characteristics of Biofilms Formed byJournal of fungi (Basel, Switzerland) · 2022Article
- Fluconazole-resistantFrontiers in fungal biology · 2022Review
- General hospital outbreak of invasive candidiasis due to azole-resistant Candida parapsilosis associated with an Erg11 Y132F mutation.Medical mycology · 2021Observational
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Among the Candida species causing bloodstream infections, Candida parapsilosis is one of the most frequently isolated. The objective of the present work was the identification of new microsatellite loci able to distinguish among C. parapsilosis isolates. DNA sequences with trinucleotide repeats were selected from the C. parapsilosis genome database. PCR primer sets flanking the microsatellite repeats were designed and tested with 20 independent isolates. On the basis of the amplification efficiency, specificity, and observed polymorphism, four of the sequences were selected for strain typing. Two hundred thirty-three independent C. parapsilosis sensu stricto isolates were genotyped by using these markers. The polymorphic loci exhibited from 20 to 42 alleles and 39 to 92 genotypes. In a multiplex analysis, 192 genotypes were obtained and the combined discriminatory power of the four microsatellites was 0.99. Reproducibility was demonstrated by submission of subcultures of 4 isolates each, in triplicate, interspersed with unique numbers among a group of 30 isolates for blind testing. Comparison of the genotypes obtained by microsatellite analysis and those obtained by randomly amplified polymorphic DNA analysis, restriction fragment length polymorphism analysis, and internal transcribed sequence grouping was performed and showed that the microsatellite method could distinguish individual isolates; none of the other methods could do that. Related species, C. orthopsilosis and C. metapsilosis, were not confused with C. parapsilosis sensu stricto. These new microsatellites are a valuable tool for use for the differentiation of C. parapsilosis sensu stricto strains, vital in epidemiology to answer questions of strain relatedness and determine pathways of transmission.
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