Evidence map›Paper›PMID 20219920›Full record

ArticleJournal of virology2010

Human papillomaviruses modulate expression of microRNA 203 upon epithelial differentiation to control levels of p63 proteins.

Marta Melar-New, Laimonis A Laimins

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.

0numbers the graph read from it
0cells of the map it votes in
108citing papers in PubMed
6.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

108 citing papers in PubMed, 171 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Review
  11. Article
  12. Epigenetic approaches for cervical neoplasia screening (Review).Experimental and therapeutic medicine · 2021
    Review
  13. Review
  14. Review
  15. Review
  16. Review
  17. Review
  18. COVID-19: fighting the invisible enemy with microRNAs.Expert review of anti-infective therapy · 2021
    Review
  19. Article
  20. Review

48 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Marta Melar-NewDepartment of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.
Laimonis A Laimins
Northwestern University · US

Funding

REGULATION OF HUMAN PAPILLOMAVIRUS GENE EXPRESSIONR01CA059655 · NCI · UNIVERSITY OF CHICAGO · PI LAIMINS, LAIMONIS A. · 1993 to 2023
$7.4M
NCI NIH HHS R01 CA059655NCI NIH HHS R01CA 59655
6 · The paper itself

Abstract

Human papillomaviruses (HPV) link their life cycles to epithelial differentiation and induce productive replication of viral DNA in suprabasal cells. Viral-DNA amplification requires cells to remain active in the cell cycle upon differentiation. This is in contrast to normal cells, which lose proliferative capability upon differentiation. One factor that negatively regulates proliferative capability upon differentiation is microRNA 203 (miR-203), which is expressed primarily in suprabasal epithelial cells. Although HPVs do not encode their own microRNAs (miRNAs), they modulate expression of cellular miRNAs to regulate the activities of cellular proteins. We show that the HPV E7 protein downregulates miR-203 expression upon differentiation, which may occur through the mitogen-activated protein (MAP) kinase/protein kinase C (PKC) pathway. One target of miR-203 is the p63 family of transcription factors, and we demonstrate that HPV-positive cells maintain significantly higher levels of these factors upon differentiation than do normal keratinocytes. Several downstream targets of p63, CARM-1, p21, and Bax, were also increased in E7-expressing cells, and their levels were inversely correlated with amounts of miR-203. Introduction of expression vectors for miR-203 into keratinocytes that stably maintain HPV episomes resulted in short-term elevation of HPV genome copy numbers, but these were rapidly lost upon subsequent passage. When HPV-positive cells expressing high levels of miR-203 were induced to differentiate in methylcellulose, impaired genome amplification was observed. We conclude that high levels of miR-203 are inhibitory to HPV amplification and that HPV proteins act to suppress expression of this microRNA to allow productive replication in differentiating cells.

Indexed as

Host-Pathogen Interactionsbcl-2-Associated X ProteinCells, CulturedCyclin-Dependent Kinase Inhibitor p21HumansKeratinocytesMicroRNAsPapillomaviridaePapillomavirus E7 ProteinsProtein-Arginine N-MethyltransferasesTrans-ActivatorsTranscription FactorsTumor Suppressor ProteinsBAX protein, humanbcl-2-Associated X ProteinCDKN1A protein, humancoactivator-associated arginine methyltransferase 1Cyclin-Dependent Kinase Inhibitor p21MicroRNAsMIRN203 microRNA, humanPapillomavirus E7 ProteinsProtein-Arginine N-MethyltransferasesTP63 protein, humanTrans-ActivatorsTranscription FactorsTumor Suppressor Proteins

Identifiers

PMID20219920
PMCPMC2863797
OpenAlexW2161581661

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.