ArticleReproductive biology and endocrinology : RB&E2010
Effect of adiponectin on bovine granulosa cell steroidogenesis, oocyte maturation and embryo development.
Article in Reproductive biology and endocrinology : RB&E, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 139 citations in OpenAlex.
- Adropin effects on granulosa cell function and oocyte maturation in pigs†.Biology of reproduction · 2026Article
- Regulatory Roles of Adiponectin in Animal Reproduction: Molecular Insights from the Hypothalamic-Pituitary-Gonadal Axis.Biomolecules · 2026Review
- New aspect on the regulation of in vitro oocyte maturation: role of the obesity, neuropeptides and adipokines.Journal of assisted reproduction and genetics · 2025Review
- A Functional 67-bp Duplication Locating at the Core Promoter Region within the BovineAnimals : an open access journal from MDPI · 2024Article
- ADPN Regulates Oxidative Stress-Induced Follicular Atresia in Geese by Modulating Granulosa Cell Apoptosis and Autophagy.International journal of molecular sciences · 2024Article
- Metabolic hormones are integral regulators of female reproductive health and function.Bioscience reports · 2024Review
- Spexin role in human granulosa cells physiology and PCOS: expression and negative impact on steroidogenesis and proliferation†.Biology of reproduction · 2023Article
- Expression of Locally Produced Adipokines and Their Receptors during Different Physiological and Reproductive Stages in the Bovine Corpus Luteum.Animals : an open access journal from MDPI · 2023Article
- Article
- Short communication: Photoperiod impacts ovarian extracellular matrix and metabolic gene expression in Siberian hamsters.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2022Article
- Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (International journal of molecular sciences · 2022Article
- Review
- Adiponectin Influences FGF2 in the Developing Porcine Corpus Luteum.Veterinary sciences · 2022Article
- Revisiting the Impact of Local Leptin Signaling in Folliculogenesis and Oocyte Maturation in Obese Mothers.International journal of molecular sciences · 2021Review
- Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways.International journal of molecular sciences · 2020Article
- Adipokines as biomarkers of postpartum subclinical endometritis in dairy cows.Reproduction (Cambridge, England) · 2020Article
- Destination of corpus luteum in postpartum clinical endometritis cows and factors affecting self-recovery.Veterinary and animal science · 2020Article
- Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A†.Biology of reproduction · 2020Article
- Adipokines Expression and Effects in Oocyte Maturation, Fertilization and Early Embryo Development: Lessons from Mammals and Birds.International journal of molecular sciences · 2020Review
- Apelin abrogates the stimulatory effects of 17β-estradiol and insulin-like growth factor-1 on proliferation of epithelial and granulosa ovarian cancer cell lines via crosstalk between APLNR and ERα/IGF1R.Molecular biology reports · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAdiponectin is an adipokine, mainly produced by adipose tissue. It regulates several reproductive processes. The protein expression of the adiponectin system (adiponectin, its receptors, AdipoR1 and AdipoR2 and the APPL1 adaptor) in bovine ovary and its role on ovarian cells and embryo, remain however to be determined.
methodsHere, we identified the adiponectin system in bovine ovarian cells and embryo using RT-PCR, immunoblotting and immunohistochemistry. Furthermore, we investigated in vitro the effects of recombinant human adiponectin (10 micro g/mL) on proliferation of granulosa cells (GC) measured by [3H] thymidine incorporation, progesterone and estradiol secretions measured by radioimmunoassay in the culture medium of GC, nuclear oocyte maturation and early embryo development.
resultsWe show that the mRNAs and proteins for the adiponectin system are present in bovine ovary (small and large follicles and corpus luteum) and embryo. Adiponectin, AdipoR1 and AdipoR2 were more precisely localized in oocyte, GC and theca cells. Adiponectin increased IGF-1 10(-8) M-induced GC proliferation (P < 0.01) but not basal or insulin 10(-8) M-induced proliferation. Additionally, adiponectin decreased insulin 10(-8) M-induced, but not basal or IGF-1 10(-8) M-induced secretions of progesterone (P < 0.01) and estradiol (P < 0.05) by GC. This decrease in insulin-induced steroidogenesis was associated with a decrease in ERK1/2 MAPK phosphorylation in GC pre-treated with adiponectin. Finally, addition of adiponectin during in vitro maturation affected neither the percentage of oocyte in metaphase-II nor 48-h cleavage and blastocyst day 8 rates.
conclusionsIn bovine species, adiponectin decreased insulin-induced steroidogenesis and increased IGF-1-induced proliferation of cultured GC through a potential involvement of ERK1/2 MAPK pathway, whereas it did not modify oocyte maturation and embryo development in vitro.
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