ArticleActa veterinaria Scandinavica2010
Comparison of variable-number tandem-repeat markers typing and IS1245 restriction fragment length polymorphism fingerprinting of Mycobacterium avium subsp. hominissuis from human and porcine origins.
Article in Acta veterinaria Scandinavica, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 37 citations in OpenAlex.
- Genotyping and drug susceptibility patterns of Mycobacterium avium subsp. hominissuis isolates from different hosts.Scientific reports · 2025Article
- Mycobacteriosis in slaughter pigs from South Africa from 1991 to 2002:Frontiers in microbiology · 2023Article
- Strain diversity inFrontiers in veterinary science · 2023Article
- Mycobacteriosis in Various Pet and Wild Birds from Germany: Pathological Findings, Coinfections, and Characterization of Causative Mycobacteria.Microbiology spectrum · 2022Article
- MIRU-VNTR Typing of Atypical Mycobacteria Isolated from the Lymph Nodes of Slaughtered Pigs from Poland.Pathogens (Basel, Switzerland) · 2022Article
- Differential Genotyping ofMicroorganisms · 2020Review
- Genotyping of swine Mycobacterium avium subsp. hominissuis isolates from Kyushu, Japan.The Journal of veterinary medical science · 2019Article
- Molecular Characterization ofFrontiers in veterinary science · 2018Article
- Comparative genome analyses of Mycobacterium avium reveal genomic features of its subspecies and strains that cause progression of pulmonary disease.Scientific reports · 2017Article
- Infection Sources of a Common Non-tuberculous Mycobacterial Pathogen,Frontiers in medicine · 2017Review
- Article
- Tuberculosis in swine co-infected with Mycobacterium avium subsp. hominissuis and Mycobacterium bovis in a cluster from Argentina.Epidemiology and infection · 2015Article
- Characterization of a novel plasmid, pMAH135, from Mycobacterium avium subsp. hominissuis.PloS one · 2015Article
- Novel insights into transmission routes of Mycobacterium avium in pigs and possible implications for human health.Veterinary research · 2014Article
- Mycobacterium avium subsp. hominissuis infection in swine associated with peat used for bedding.BioMed research international · 2014Article
- Quantification of Mycobacterium avium subspecies in pig tissues by real-time quantitative PCR.Acta veterinaria Scandinavica · 2013Article
- Correlation between variable-number tandem-repeat-based genotypes and drug susceptibility in Mycobacterium avium isolates.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundAnimal mycobacterioses are regarded as a potential zoonotic risk and cause economical losses world wide. M. avium subsp. hominissuis is a slow-growing subspecies found in mycobacterial infected humans and pigs and therefore rapid and discriminatory typing methods are needed for epidemiological studies. The genetic similarity of M. avium subsp. hominissuis from human and porcine origins using two different typing methods have not been studied earlier. The objective of this study was to compare the IS1245 RFLP pattern and MIRU-VNTR typing to study the genetic relatedness of M. avium strains isolated from slaughter pigs and humans in Finland with regard to public health aspects.
methodsA novel PCR-based genotyping method, variable number tandem repeat (VNTR) typing of eight mycobacterial interspersed repetitive units (MIRUs), was evaluated for its ability to characterize Finnish Mycobacterium avium subsp. hominissuis strains isolated from pigs (n = 16) and humans (n = 13) and the results were compared with those obtained by the conventional IS1245 RFLP method.
resultsThe MIRU-VNTR results showed a discriminatory index (DI) of 0,92 and the IS1245 RFLP resulted in DI 0,98. The combined DI for both methods was 0,98. The MIRU-VNTR test has the advantages of being simple, reproducible, non-subjective, which makes it suitable for large-scale screening of M. avium strains.
conclusionsBoth typing methods demonstrated a high degree of similarity between the strains of human and porcine origin. The parallel application of the methods adds epidemiological value to the comparison of the strains and their origins. The present approach and results support the hypothesis that there is a common source of M. avium subsp. hominissuis infection for pigs and humans or alternatively one species may be the infective source to the other.
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