ReviewRecent patents on DNA & gene sequences2010
Modulation of ColE1-like plasmid replication for recombinant gene expression.
Review in Recent patents on DNA & gene sequences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Multi-gene Co-expression systems inSynthetic and systems biotechnology · 2026Review
- CRISPR-associated transposon for programmable viral vector engineering and prime editing.Nucleic acids research · 2026Article
- Generating functional plasmid origins with OriGen.Nucleic acids research · 2025Article
- Article
- Deciphering plasmid replication dynamics: a computational approach to predict ColE1-like plasmid copy number.Journal of the Royal Society, Interface · 2025Article
- Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts:Chemical reviews · 2025Review
- Deletion of pcnB affects antibiotic susceptibility in resistant Escherichia coli by reducing copy number of ColE1-family plasmids.Scientific reports · 2025Article
- Dynamic control of the plasmid copy number maintained without antibiotics in Escherichia coli.Journal of biological engineering · 2024Article
- Stochastic nature and physiological implications of 5'-NAD RNA cap in bacteria.Nucleic acids research · 2024Article
- Engineering plasmid copy number heterogeneity for dynamic microbial adaptation.Nature microbiology · 2024Article
- The enhancement of M13 phage titration by optimizing the origin of replication.Research in pharmaceutical sciences · 2024Article
- Optimized transgene expression in the red alga Porphyridium purpureum and efficient recombinant protein secretion into the culture medium.Plant molecular biology · 2024Article
- System-wide optimization of an orthogonal translation system with enhanced biological tolerance.Molecular systems biology · 2023Article
- Article
- Factors involved in heterologous expression of proteins in E. coli host.Archives of microbiology · 2023Review
- Development of high-copy number plasmids in Pseudoalteromonas haloplanktis TAC125.Applied microbiology and biotechnology · 2023Article
- Molecular Characterization of a DNA Polymerase fromInternational journal of molecular sciences · 2022Article
- A plasmid system with tunable copy number.Nature communications · 2022Article
- Harnessing plasmid replication mechanism to enable dynamic control of gene copy in bacteria.Metabolic engineering · 2022Article
- Evolution of ColE1-like plasmids across γ-Proteobacteria: From bacteriocin production to antimicrobial resistance.PLoS genetics · 2021Article
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Funding
Abstract
ColE1-like plasmids constitute the most popular vectors for recombinant protein expression. ColE1 plasmid replication is tightly controlled by an antisense RNA mechanism that is highly dynamic, tuning plasmid metabolic burden to the physiological state of the host. Plasmid homeostasis is upset upon induction of recombinant protein expression because of non-physiological levels of expression and because of the frequently biased amino acid composition of recombinant proteins. Disregulation of plasmid replication is the main cause of collapse of plasmid-based expression systems because of a simultaneous increase in the metabolic burden (due to increased average copy number) and in the probability of generation of plasmid-free cells (due to increased copy number variation). Interference between regulatory elements of co-resident plasmids causes comparable effects on plasmid stability (plasmid incompatibility). Modulating plasmid copy number for recombinant gene expression aims at achieving a high gene dosage while preserving the stability of the expression system. Here I present strategies targeting plasmid replication for optimizing recombinant gene expression. Specifically, I review approaches aimed at modulating the antisense regulatory system (as well as their implications for plasmid incompatibility) and innovative strategies involving modulation of host factors, of R-loop formation, and of the timing of recombinant gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.