ArticleJournal of agricultural and food chemistry2010
Using confocal laser scanning microscopy to probe the milk fat globule membrane and associated proteins.
Article in Journal of agricultural and food chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 208 citations in OpenAlex.
- Milk phospholipid-coated lipid droplets modulate the infant gut microbiota and metabolome influencing weight gain.Microbiome · 2025Trial
- Formula with large, milk phospholipid-coated lipid droplets in late-moderate preterm infants: a double-blind RCT.Pediatric research · 2025Trial
- Trial
- An infant formula with large, milk phospholipid-coated lipid droplets containing a mixture of dairy and vegetable lipids supports adequate growth and is well tolerated in healthy, term infants.The American journal of clinical nutrition · 2019Trial
- Distinct Temporal Mouthfeel in Dairy and Plant-Based Milk Is Related to Their Rheological and Tribological Spectra.Journal of texture studies · 2026Article
- Deep Learning-Assisted Microscopy Reveals Progressive Supramolecular Remodeling and Colloidal Reorganization of Bovine Milk Induced by Centrifugation.International journal of molecular sciences · 2026Article
- Article
- Genomic characterization of normal and aberrant human milk production.Science advances · 2025Article
- The impact of sphingomyelin and cholesterol on ordered lipid domain formation in the bovine milk fat globule membrane using artificial giant unilamellar vesicles as a model.JDS communications · 2025Article
- Composition and structural characteristics of lipids in yak, buffalo, and cow colostrum based on untargeted lipidomics.NPJ science of food · 2025Article
- Addition of Phospholipids Improved the Physical Stability and Fat Globule Structure of Processed Milk.Foods (Basel, Switzerland) · 2025Article
- Refined tamoxifen administration in mice by encouraging voluntary consumption of palatable formulations.Lab animal · 2024Article
- Analysis of Milk Microstructure Using Raman Hyperspectral Imaging.Molecules (Basel, Switzerland) · 2023Article
- Beyond ingredients: Supramolecular structure of lipid droplets in infant formula affects metabolic and brain function in mouse models.PloS one · 2023Article
- Brain-immune-gut benefits with early life supplementation of milk fat globule membrane.JGH open : an open access journal of gastroenterology and hepatology · 2022Review
- Comparative Structural and Compositional Analyses of Cow, Buffalo, Goat and Sheep Cream.Foods (Basel, Switzerland) · 2021Article
- An Observational Cohort Study and Nested Randomized Controlled Trial on Nutrition and Growth Outcomes in Moderate and Late Preterm Infants (FLAMINGO).Frontiers in nutrition · 2021Article
- Combination of High Hydrostatic Pressure and Ultrafiltration to Generate a New Emulsifying Ingredient from Egg Yolk.Molecules (Basel, Switzerland) · 2020Article
- Nature-Assembled Structures for Delivery of Bioactive Compounds and Their Potential in Functional Foods.Frontiers in chemistry · 2020Review
- Characterization of difference in structure and function of fresh and mastitic bovine milk fat globules.PloS one · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The bovine milk fat globule membrane (MFGM) is an important, biologically relevant membrane due to its functional and health properties. Its composition has been thoroughly studied, but its structure, especially the lateral organization of its components, still remains unclear. We have used confocal laser scanning microscopy (CLSM) to investigate the surface structure of the MFGM in globules with different degrees of processing using two types of fluorescently labeled phospholipid probes and a protein dye. Using this technique, we have observed heterogeneities in the distribution of MFGM lipids and proteins relating to the processing and size of the globules. The effect of pretreating the milk (centrifugation, pasteurization-homogenization and churning) was studied by double-staining the surface of the milk fat globules, followed by observation using CLSM, and by determining the phospholipid profile of raw milk, raw cream, processed milk and buttermilk powder. Our findings agree with other techniques by showing that the composition of the MFGM changes with processing through the loss of phospholipids and the adsorption of caseins and whey proteins onto the surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.