ArticleAnalytical chemistry2010
Enrichment and detection of Escherichia coli O157:H7 from water samples using an antibody modified microfluidic chip.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 102 citations in OpenAlex.
- UV-Vis detection ofMethodsX · 2024Article
- Polymeric and Paper-Based Lab-on-a-Chip Devices in Food Safety: A Review.Micromachines · 2023Review
- Flow-Injection Methods in Water Analysis-Recent Developments.Molecules (Basel, Switzerland) · 2022Review
- Advances in Plant Disease Detection and Monitoring: From Traditional Assays to In-Field Diagnostics.Sensors (Basel, Switzerland) · 2021Review
- A Comprehensive Review of Microfluidic Water Quality Monitoring Sensors.Sensors (Basel, Switzerland) · 2019Review
- Microfluidics-Based Organism Isolation from Whole Blood: An Emerging Tool for Bloodstream Infection Diagnosis.Annals of biomedical engineering · 2019Review
- WaterSpy: A High Sensitivity, Portable Photonic Device for Pervasive Water Quality Analysis.Sensors (Basel, Switzerland) · 2018Article
- Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology.Scientific reports · 2018Article
- Novel Microfluidic Analytical Sensing Platform for the Simultaneous Detection of Three Algal Toxins in Water.ACS omega · 2018Article
- Article
- Optofluidic Technology for Water Quality Monitoring.Micromachines · 2018Review
- Large-Area and High-Throughput PDMS Microfluidic Chip Fabrication Assisted by Vacuum Airbag Laminator.Micromachines · 2017Article
- Review: Microbial analysis in dielectrophoretic microfluidic systems.Analytica chimica acta · 2017Review
- Advanced biosensors for detection of pathogens related to livestock and poultry.Veterinary research · 2017Review
- Most probable number - loop mediated isothermal amplification (MPN-LAMP) for quantifying waterborne pathogens in <25min.Journal of microbiological methods · 2017Article
- A review on detection methods used for foodborne pathogens.The Indian journal of medical research · 2016Review
- Fabrication of Polymerase Chain Reaction Plastic Lab-on-a-Chip Device for Rapid Molecular Diagnoses.International neurourology journal · 2016Article
- Signal-off impedimetric immunosensor for the detection of Escherichia coli O157:H7.Scientific reports · 2016Article
- Nano-capillary electrophoresis for environmental analysis.Environmental chemistry letters · 2016Review
- A portable automatic endpoint detection system for amplicons of loop mediated isothermal amplification on microfluidic compact disk platform.Sensors (Basel, Switzerland) · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Low abundant (<100 cells mL(-1)) E. coli O157:H7 cells were isolated and enriched from environmental water samples using a microfluidic chip. The poly(methylmethacrylate), PMMA, chip contained 8 devices, each equipped with 16 curvilinear high aspect ratio channels that were covalently decorated with polyclonal anti-O157 antibodies (pAb) and could search for rare cells through a pAb mediated process. The chip could process independently 8 different samples or one sample using 8 different parallel inputs to increase volume processing throughput. After cell enrichment, cells were released and enumerated using benchtop real-time quantitative polymerase chain reaction (PCR), targeting genes which effectively discriminated the O157:H7 serotype from other nonpathogenic bacteria. The recovery of target cells from water samples was determined to be approximately 72%, and the limit-of-detection was found to be 6 colony forming units (cfu) using the slt1 gene as a reporter. We subsequently performed analysis of lake and wastewater samples. The simplicity in manufacturing and ease of operation makes this device attractive for the selection of pathogenic species from a variety of water supplies suspected of containing bacterial pathogens at extremely low frequencies.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.