ArticleProteomics2010
Descriptive proteomic analysis shows protein variability between closely related clinical isolates of Mycobacterium tuberculosis.
Article in Proteomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Dynamic Measurement of Protein Translation in Mycobacteria Using Nontargeted Stable Isotope Labeling in Combination with MALDI-TOF Mass Spectrometry-Based Readout.Analytical chemistry · 2025Article
- Identification and architecture of a putative secretion tube across mycobacterial outer envelope.Science advances · 2021Article
- Methods for Proteomic Analyses of Mycobacteria.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides.Scientific reports · 2020Article
- Second generation multiple reaction monitoring assays for enhanced detection of ultra-low abundanceClinical proteomics · 2017Article
- Comparative Proteomic Analyses of Avirulent, Virulent, and Clinical Strains of Mycobacterium tuberculosis Identify Strain-specific Patterns.The Journal of biological chemistry · 2016Article
- Proteomics Analysis of Three Different Strains of Mycobacterium tuberculosis under In vitro Hypoxia and Evaluation of Hypoxia Associated Antigen's Specific Memory T Cells in Healthy Household Contacts.Frontiers in microbiology · 2016Article
- Article
- Hands-on workshops as an effective means of learning advanced technologies including genomics, proteomics and bioinformatics.Genomics, proteomics & bioinformatics · 2013Article
- Visualization of imbalances in sulfur assimilation and synthesis of sulfur-containing amino acids at the single-cell level.Applied and environmental microbiology · 2013Article
- Analysis of the secretome and identification of novel constituents from culture filtrate of bacillus Calmette-Guerin using high-resolution mass spectrometry.Molecular & cellular proteomics : MCP · 2013Article
- Upregulation of the phthiocerol dimycocerosate biosynthetic pathway by rifampin-resistant, rpoB mutant Mycobacterium tuberculosis.Journal of bacteriology · 2012Article
- Use of recombinant purified protein derivative (PPD) antigens as specific skin test for tuberculosis.The Indian journal of medical research · 2012Article
- Sample preparation of Mycobacterium tuberculosis extracts for nuclear magnetic resonance metabolomic studies.Journal of visualized experiments : JoVE · 2012Article
- Deciphering the proteome of the in vivo diagnostic reagent "purified protein derivative" from Mycobacterium tuberculosis.Proteomics · 2012Article
- Epidemiologic consequences of microvariation in Mycobacterium tuberculosis.The Journal of infectious diseases · 2012Article
- Analysis of intracellular expressed proteins of Mycobacterium tuberculosis clinical isolates.Proteome science · 2012Article
- Three protein cocktails mediate delayed-type hypersensitivity responses indistinguishable from that elicited by purified protein derivative in the guinea pig model of Mycobacterium tuberculosis infection.Infection and immunity · 2011Article
- Article
- Proteomic definition of the cell wall of Mycobacterium tuberculosis.Journal of proteome research · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The use of isobaric tags such as iTRAQ allows the relative and absolute quantification of hundreds of proteins in a single experiment for up to eight different samples. More classical techniques such as 2-DE can offer a complimentary approach for the analysis of complex protein samples. In this study, the proteomes of secreted and cytosolic proteins of genetically closely related strains of Mycobacterium tuberculosis were analyzed. Analysis of 2-D gels afforded 28 spots with variations in protein abundance between strains. These were identified by MS/MS. Meanwhile, a rigorous statistical analysis of iTRAQ data allowed the identification and quantification of 101 and 137 proteins in the secreted and cytosolic fractions, respectively. Interestingly, several differences in protein levels were observed between the closely related strains BE, C28 and H6. Seven proteins related to cell wall and cell processes were more abundant in BE, while enzymes related to metabolic pathways (GltA2, SucC, Gnd1, Eno) presented lower levels in the BE strain. Proteins involved in iron and sulfur acquisition (BfrB, ViuB, TB15.3 and SseC2) were more abundant in C28 and H6. In general, iTRAQ afforded rapid identification of fine differences between protein levels such as those presented between closely related strains. This provides a platform from which the relevance of these differences can be assessed further using complimentary proteomic and biological modeling methods.
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Registered trials
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