ArticleMethods in molecular biology (Clifton, N.J.)2010
A capture coupling method for the covalent immobilization of hexahistidine tagged proteins for surface plasmon resonance.
Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
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- Characterization of high-affinity antibodies against the surface Gc protein ofBiochemistry and biophysics reports · 2024Article
- A Simple and Versatile Strategy for Oriented Immobilization of His-Tagged Proteins on Magnetic Nanoparticles.Bioconjugate chemistry · 2023Article
- Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics.Sensors (Basel, Switzerland) · 2021Review
- Dynamics of the HD regulatory subdomain of PARP-1; substrate access and allostery in PARP activation and inhibition.Nucleic acids research · 2021Article
- Evaluation of Computationally Designed Peptides against TWEAK, a Cytokine of the Tumour Necrosis Factor Ligand Family.International journal of molecular sciences · 2021Article
- Molecular basis for protein-protein interactions.Beilstein journal of organic chemistry · 2021Review
- Monitoring bioactive and total antibody concentrations for continuous process control by surface plasmon resonance spectroscopy.Engineering in life sciences · 2019Article
- Selection and Characterization of Monoclonal Antibodies Targeting Middle East Respiratory Syndrome Coronavirus through a Human Synthetic Fab Phage Display Library Panning.Antibodies (Basel, Switzerland) · 2019Article
- Two Regulators, PA3898 and PA2100, Modulate the Pseudomonas aeruginosa Multidrug Resistance MexAB-OprM and EmrAB Efflux Pumps and Biofilm Formation.Antimicrobial agents and chemotherapy · 2018Article
- Novel dual regulators of Pseudomonas aeruginosa essential for productive biofilms and virulence.Molecular microbiology · 2018Article
- Quantifying the relationship between inhibition of VEGF receptor 2, drug-induced blood pressure elevation and hypertension.British journal of pharmacology · 2018Article
- Thermo-kinetic analysis space expansion for cyclophilin-ligand interactions - identification of a new nonpeptide inhibitor using Biacore™ T200.FEBS open bio · 2017Article
- Crystal structure of cGMP-dependent protein kinase Iβ cyclic nucleotide-binding-B domain : Rp-cGMPS complex reveals an apo-like, inactive conformation.FEBS letters · 2017Article
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- Characterization of heme binding to recombinant α1-microglobulin.Frontiers in physiology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Surface plasmon resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one component must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thiols, or noncovalent immobilization using high-affinity interactions such as biotin/streptavidin or antibody/antigen. In this chapter we describe a robust method to covalently immobilize His(6) fusion proteins on the sensor surface for SPR analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.