ArticleMethods in molecular biology (Clifton, N.J.)2010
High-affinity immobilization of proteins using biotin- and GST-based coupling strategies.
Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
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- Molecular Modeling and In Vitro Functional Analysis of the RGS12 PDZ Domain Variant Associated with High-Penetrance Familial Bipolar Disorder.International journal of molecular sciences · 2024Article
- Surface Plasmon Resonance: A Sensitive Tool to Study Protein-Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Extract2Chip-Bypassing Protein Purification in Drug Discovery Using Surface Plasmon Resonance.Biosensors · 2023Article
- Grafting Strategies of Oxidation-Prone Coiled-Coil Peptides for Protein Capture in Bioassays: Impact of Orientation and the Oxidation State.ACS omega · 2023Article
- Numerical analysis of hafnium oxide and phase change material-based multi-layered infrared and visible frequency sensor for biomolecules sensing application.Scientific reports · 2023Article
- Modification of Cysteine-Substituted Antibodies Using Enzymatic Oxidative Coupling Reactions.Bioconjugate chemistry · 2023Article
- Characterization of Small Molecule-Protein Interactions Using SPR Method.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Geometric Control of Cell Behavior by Biomolecule Nanodistribution.ACS biomaterials science & engineering · 2022Article
- NIN-like protein 7 transcription factor is a plant nitrate sensor.Science (New York, N.Y.) · 2022Article
- Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics.Sensors (Basel, Switzerland) · 2021Review
- Tunable infrared metamaterial-based biosensor for detection of hemoglobin and urine using phase change material.Scientific reports · 2021Article
- Nanotechnology-Based Strategies to Develop New Anticancer Therapies.Biomolecules · 2020Review
- Article
- In Vitro Analytical Approaches to Study Plant Ligand-Receptor Interactions.Plant physiology · 2020Review
- Secondary modification of oxidatively-modified proline N-termini for the construction of complex bioconjugates.Organic & biomolecular chemistry · 2020Article
- A role for Regulator of G protein Signaling-12 (RGS12) in the balance between myoblast proliferation and differentiation.PloS one · 2019Article
- Functional expression of monomeric streptavidin and fusion proteins in Escherichia coli: applications in flow cytometry and ELISA.Applied microbiology and biotechnology · 2018Article
- Surface Plasmon Resonance kinetic analysis of the interaction between G-quadruplex nucleic acids and an anti-G-quadruplex monoclonal antibody.Biochimica et biophysica acta. General subjects · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.