ReviewMethods in molecular biology (Clifton, N.J.)2010
The role of mass transport limitation and surface heterogeneity in the biophysical characterization of macromolecular binding processes by SPR biosensing.
Review in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed.
- Characterizing Variations in Binding Kinetics of Affinity Biosensors: A Boundary-Layer and Site-Quality Approach.ACS omega · 2026Article
- Clocked stepping of an artificial protein walker along a DNA track.Nature nanotechnology · 2026Article
- Dissecting mechanisms of ligand binding and conformational changes in the glutamine-binding protein.eLife · 2026Article
- Non-enzymatic dual-mode plasmonic framework for robust bioanalyte detection.Scientific reports · 2026Article
- A PKA-selective inhibitor captures an open but more ordered conformation of the PKA catalytic subunit.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Development of VP30-targeted nanoparticles using DPS4 fusion peptides for the inhibition of Ebola virus.Journal of nanobiotechnology · 2026Article
- Probing Glycosaminoglycan-Protein Interactions: Applications of Surface Plasmon Resonance.Biosensors · 2026Review
- Interrogation of Small Molecules to Surface-Bound Aptamer Binding Kinetics with Electrochemical Aptamer-Based Sensors Using Intermittent Pulse Amperometry.Analytical chemistry · 2025Article
- Sheet Protector Strategy for Western Blot to Reduce Antibody Consumption and Incubation Time.Biological procedures online · 2025Article
- In-Solution Characterization of Biomolecular Interaction Kinetics under Native Conditions.Analytical chemistry · 2025Article
- PCANN Program for Structure-Based Prediction of Protein-Protein Binding Affinity: Comparison With Other Neural-Network Predictors.Proteins · 2025Article
- High-throughput investigation of macromolecular interactions for drug development using spectral shift technology.Biophysical reviews · 2025Review
- Identification and Validation of Cyclic Peptides with Mucin-Selective, Location-Specific Binding in the Gastrointestinal Tract.ACS nano · 2025Article
- Modular Nanoparticle Platform for Solution-Phase Optical Sensing of Protein-Protein Interactions.ACS applied optical materials · 2025Article
- Decoding aptamer-protein binding kinetics for continuous biosensing using single-molecule techniques.Science advances · 2025Article
- In vivo brain delivery of BBB-enabled iduronate 2-sulfatase in rats.Fluids and barriers of the CNS · 2025Article
- Applications of Surface Plasmon Resonance for Advanced Studies Involving Nucleic Acids.RNA nanomed · 2024Article
- Effects of Cosolvent on the Intermolecular Interactions between an Analyte and a Gold Nanostar Surface Studied Using SERS.The journal of physical chemistry. C, Nanomaterials and interfaces · 2024Article
- Antibody-independent surface plasmon resonance assays for influenza vaccine quality control.Applied microbiology and biotechnology · 2024Review
- Thermophoretic glycan profiling of extracellular vesicles for triple-negative breast cancer management.Nature communications · 2024Article
27 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors.
Funding
Abstract
This chapter presents an introduction to the kinetic analysis of SPR biosensor data for the determination of affinity and kinetic rate constants of biomolecular interactions between an immobilized and a soluble binding partner. The need to be aware of and critically test the assumptions underlying the analysis models is emphasized and the consequences for the experimental design are discussed. The two most common sources of deviation in SPR surface binding kinetics from the ideal pseudo-first-order binding kinetics of bimolecular reactions are mass transport limitations and the heterogeneity of the surface sites. These problems are intrinsic to the use of a biosensor surface for characterizing interactions. The effect of these factors on the observed binding kinetics, and strategies to account for them are reviewed, both in the context of mathematical data analysis, as well as the design of the experiments and controls.
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