ArticleMolecular therapy : the journal of the American Society of Gene Therapy2010
Particle tracking of intracellular trafficking of octaarginine-modified liposomes: a comparative study with adenovirus.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Sequence-defined polymers for predictable gene delivery.Nature reviews. Chemistry · 2026Review
- Anticodon-edited transfer RNAs (ACE-tRNAs) encoded as therapeutic nonviral minimal DNA vectors.Nucleic acids research · 2026Article
- Anticodon Edited Transfer RNAs (ACE-tRNAs) Encoded as Therapeutic Nonviral Minimal DNA Vectors.bioRxiv : the preprint server for biology · 2025Article
- Influence of the chirality of carbon nanodots on their interaction with proteins and cells.Nature communications · 2021Article
- Synthetic Approaches for Nucleic Acid Delivery: Choosing the Right Carriers.Life (Basel, Switzerland) · 2019Review
- Endocytic uptake of monomeric amyloid-β peptides is clathrin- and dynamin-independent and results in selective accumulation of Aβ(1-42) compared to Aβ(1-40).Scientific reports · 2017Article
- Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles.Acta biomaterialia · 2016Article
- Gene Electrotransfer: A Mechanistic Perspective.Current gene therapy · 2016Review
- Particle tracking in drug and gene delivery research: State-of-the-art applications and methods.Advanced drug delivery reviews · 2015Review
- Intracellular tracking of single-plasmid DNA particles after delivery by electroporation.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Investigation of Polyethylenimine/DNA Polyplex Transfection to Cultured Cells Using Radiolabeling and Subcellular Fractionation Methods.Molecular pharmaceutics · 2013Article
- The role of cytoskeleton networks on lipid-mediated delivery of DNA.Therapeutic delivery · 2013Article
- Particle tracking analysis for the intracellular trafficking of nanoparticles modified with African swine fever virus protein p54-derived peptide.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Quantitative measurement of intracellular transport of nanocarriers by spatio-temporal image correlation spectroscopy.Methods and applications in fluorescence · 2013Article
- Cytoplasmic trafficking, endosomal escape, and perinuclear accumulation of adeno-associated virus type 2 particles are facilitated by microtubule network.Journal of virology · 2012Article
- The emergence of multiple particle tracking in intracellular trafficking of nanomedicines.Biophysical reviews · 2012Review
- Quantitative nanoparticle tracking: applications to nanomedicine.Nanomedicine (London, England) · 2011Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is previously reported that octaarginine (R8)-modified liposome (R8-Lip) was taken up via macropinocytosis, and subsequently delivered to the nuclear periphery. In the present study, we investigated the mechanism for the cytoplasmic transport of R8-Lips, comparing with that for adenovirus. Treatment with microtubule-disruption reagent (nocodazole) inhibited the transfection activity of plasmid DNA (pDNA)-encapsulating R8-Lip more extensively than that of adenovirus. The directional transport of R8-Lips along green fluorescent protein (GFP)-tagged microtubules was observed; however, the velocity was slower than those for adenovirus or endosomes that were devoid of R8-Lips. These directional motions were abrogated in R8-Lips by nocodazole treatment, whereas adenovirus continued to undergo random motion. This finding suggests that the nuclear access of R8-Lip predominantly involves microtubule-dependent transport, whereas an apparent diffusive motion is also operative in nuclear access of adenovirus. Furthermore, quantum dot-labeled pDNA underwent directional motion concomitantly with rhodamine-labeled lipid envelopes, indicating that the R8-Lips were subject to microtubule-dependent transport in the intact form. Dual particle tracking of carriers and endosomes revealed that R8-Lip was directionally transported, associated with endosomes, whereas this occurs after endosomal escape in adenovirus. Collectively, the findings reported herein indicate that vesicular transport is a key factor in the cytoplasmic transport of R8-Lips.
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