ArticleNucleic acids research2010
Cleavage of the sarcin-ricin loop of 23S rRNA differentially affects EF-G and EF-Tu binding.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- Key Proteins and Enzymatic Mechanisms Underlying Drug Resistance in Mycobacterium tuberculosis.Advances in experimental medicine and biology · 2026Review
- Exploring GNRA tetraloop-like motifs in nucleic acid 3D structures.Scientific reports · 2025Article
- RNAMotifProfile: a graph-based approach to build RNA structural motif profiles.NAR genomics and bioinformatics · 2024Article
- Review
- Optimization of a fluorescent-mRNA based real-time assay for precise kinetic measurements of ribosomal translocation.RNA biology · 2021Article
- RNAMotifContrast: a method to discover and visualize RNA structural motif subfamilies.Nucleic acids research · 2021Article
- Messenger RNA delivery to mitoribosomes - hints from a bacterial toxin.The FEBS journal · 2021Article
- Gene Organization, Expression, and Localization of Ribotoxin-Like Protein Ageritin in Fruiting Body and Mycelium ofInternational journal of molecular sciences · 2020Article
- The ribotoxin α-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes.Nucleic acids research · 2020Article
- Piece by piece: Building a ribozyme.The Journal of biological chemistry · 2020Review
- De novo discovery of structural motifs in RNA 3D structures through clustering.Nucleic acids research · 2018Article
- The ribosomal A-site finger is crucial for binding and activation of the stringent factor RelA.Nucleic acids research · 2018Article
- Determination of nucleoside triphosphatase activities from measurement of true inorganic phosphate in the presence of labile phosphate compounds.Analytical biochemistry · 2017Article
- Review
- A deimmunised form of the ribotoxin, α-sarcin, lacking CD4+ T cell epitopes and its use as an immunotoxin warhead.Protein engineering, design & selection : PEDS · 2016Article
- Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Role of a ribosomal RNA phosphate oxygen during the EF-G-triggered GTP hydrolysis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Efficient in vivo antitumor effect of an immunotoxin based on ribotoxin α-sarcin in nude mice bearing human colorectal cancer xenografts.SpringerPlus · 2015Article
- EF-G and EF4: translocation and back-translocation on the bacterial ribosome.Nature reviews. Microbiology · 2014Review
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribotoxins are potent inhibitors of protein biosynthesis and inactivate ribosomes from a variety of organisms. The ribotoxin alpha-sarcin cleaves the large 23S ribosomal RNA (rRNA) at the universally conserved sarcin-ricin loop (SRL) leading to complete inactivation of the ribosome and cellular death. The SRL interacts with translation factors that hydrolyze GTP, and it is important for their binding to the ribosome, but its precise role is not yet understood. We studied the effect of alpha-sarcin on defined steps of translation by the bacterial ribosome. alpha-Sarcin-treated ribosomes showed no defects in mRNA and tRNA binding, peptide-bond formation and sparsomycin-dependent translocation. Cleavage of SRL slightly affected binding of elongation factor Tu ternary complex (EF-Tu*GTP*tRNA) to the ribosome. In contrast, the activity of elongation factor G (EF-G) was strongly impaired in alpha-sarcin-treated ribosomes. Importantly, cleavage of SRL inhibited EF-G binding, and consequently GTP hydrolysis and mRNA-tRNA translocation. These results suggest that the SRL is more critical in EF-G than ternary complex binding to the ribosome implicating different requirements in this region of the ribosome during protein elongation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.