ArticleDevelopment (Cambridge, England)2010
Consistent left-right asymmetry cannot be established by late organizers in Xenopus unless the late organizer is a conjoined twin.
Article in Development (Cambridge, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Laterality defects are influenced by timing of treatments and animal model.Differentiation; research in biological diversity · 2012Pooled it
- Vertebrate Left-Right Asymmetry: What Can Nodal Cascade Gene Expression Patterns Tell Us?Journal of cardiovascular development and disease · 2017Review
- A conceptual model of morphogenesis and regeneration.Acta biotheoretica · 2015Article
- Symmetry breakage in the vertebrate embryo: when does it happen and how does it work?Developmental biology · 2014Review
- Left-right patterning in Xenopus conjoined twin embryos requires serotonin signaling and gap junctions.The International journal of developmental biology · 2014Article
- It's never too early to get it Right: A conserved role for the cytoskeleton in left-right asymmetry.Communicative & integrative biology · 2013Article
- A unified model for left-right asymmetry? Comparison and synthesis of molecular models of embryonic laterality.Developmental biology · 2013Review
- Ectopic eyes outside the head in Xenopus tadpoles provide sensory data for light-mediated learning.The Journal of experimental biology · 2013Article
- Polarity proteins are required for left-right axis orientation and twin-twin instruction.Genesis (New York, N.Y. : 2000) · 2012Article
- A framework for the establishment of a cnidarian gene regulatory network for "endomesoderm" specification: the inputs of ß-catenin/TCF signaling.PLoS genetics · 2012Article
- The multiple roles of Notch signaling during left-right patterning.Cellular and molecular life sciences : CMLS · 2011Review
- Article
- A second-generation device for automated training and quantitative behavior analyses of molecularly-tractable model organisms.PloS one · 2010Article
- Far from solved: a perspective on what we know about early mechanisms of left-right asymmetry.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
How embryos consistently orient asymmetries of the left-right (LR) axis is an intriguing question, as no macroscopic environmental cues reliably distinguish left from right. Especially unclear are the events coordinating LR patterning with the establishment of the dorsoventral (DV) axes and midline determination in early embryos. In frog embryos, consistent physiological and molecular asymmetries manifest by the second cell cleavage; however, models based on extracellular fluid flow at the node predict correct de novo asymmetry orientation during neurulation. We addressed these issues in Xenopus embryos by manipulating the timing and location of dorsal organizer induction: the primary dorsal organizer was ablated by UV irradiation, and a new organizer was induced at various locations, either early, by mechanical rotation, or late, by injection of lithium chloride (at 32 cells) or of the transcription factor XSiamois (which functions after mid-blastula transition). These embryos were then analyzed for the position of three asymmetric organs. Whereas organizers rescued before cleavage properly oriented the LR axis 90% of the time, organizers induced in any position at any time after the 32-cell stage exhibited randomized laterality. Late organizers were unable to correctly orient the LR axis even when placed back in their endogenous location. Strikingly, conjoined twins produced by late induction of ectopic organizers did have normal asymmetry. These data reveal that although correct LR orientation must occur no later than early cleavage stages in singleton embryos, a novel instructive influence from an early organizer can impose normal asymmetry upon late organizers in the same cell field.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.