ArticleThe Journal of biological chemistry2010
Light-dependent phosphorylation of the drosophila transient receptor potential ion channel.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Ultrasonic neuromodulation mediated by mechanosensitive ion channels: current and future.Frontiers in neuroscience · 2023Article
- Retinal TRP channels: Cell-type-specific regulators of retinal homeostasis and multimodal integration.Progress in retinal and eye research · 2023Review
- Functional characterization of the three Drosophila retinal degeneration C (RDGC) protein phosphatase isoforms.PloS one · 2018Article
- The latency of the light response is modulated by the phosphorylation state of Drosophila TRP at a specific site.Channels (Austin, Tex.) · 2017Article
- Phosphorylation of Suppressor of Hairless impedes its DNA-binding activity.Scientific reports · 2017Article
- The Phosphorylation State of theThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Article
- Phosphorylation of rat melanopsin at Ser-381 and Ser-398 by light/dark and its importance for intrinsically photosensitive ganglion cells (ipRGCs) cellular Ca2+ signaling.The Journal of biological chemistry · 2014Article
- Arabidopsis MSL10 has a regulated cell death signaling activity that is separable from its mechanosensitive ion channel activity.The Plant cell · 2014Article
- Article
- Proteomic survey reveals altered energetic patterns and metabolic failure prior to retinal degeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Identification and functional analysis of phosphorylation residues of the Arabidopsis BOTRYTIS-INDUCED KINASE1.Protein & cell · 2013Article
- Drosophila TRP and TRPL are assembled as homomultimeric channels in vivo.Journal of cell science · 2013Article
- Mutation of light-dependent phosphorylation sites of the Drosophila transient receptor potential-like (TRPL) ion channel affects its subcellular localization and stability.The Journal of biological chemistry · 2013Article
- Article
- Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila phototransduction cascade terminates in the opening of an ion channel, designated transient receptor potential (TRP). TRP has been shown to become phosphorylated in vitro, suggesting regulation of the ion channel through posttranslational modification. However, except for one phosphorylation site, Ser(982), which was analyzed by functional in vivo studies (Popescu, D. C., Ham, A. J., and Shieh, B. H. (2006) J. Neurosci. 26, 8570-8577), nothing is known about the role of TRP phosphorylation in vivo. Here, we report the identification of 21 TRP phosphorylation sites by a mass spectrometry approach. 20 phosphorylation sites are located in the C-terminal portion of the channel, and one site is located near the N terminus. All 21 phosphorylation sites were also identified in the inaC(P209) mutant, indicating that phosphorylation of TRP at these sites occurred independently from the eye-enriched protein kinase C. Relative quantification of phosphopeptides revealed that at least seven phosphorylation sites were predominantly phosphorylated in the light, whereas one site, Ser(936), was predominantly phosphorylated in the dark. We show that TRP phosphorylated at Ser(936) was located in the rhabomere. Light-dependent changes in the phosphorylation state of this site occurred within minutes. The dephosphorylation of TRP at Ser(936) required activation of the phototransduction cascade.
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