ArticleArthritis research & therapy2010
Automated evaluation of autoantibodies on human epithelial-2 cells as an approach to standardize cell-based immunofluorescence tests.
Article in Arthritis research & therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 112 citations in OpenAlex.
- A human epithelial cell cycle dataset.Scientific data · 2026Article
- Artificial intelligence applications for immunology laboratory: image analysis and classification study of IIF photos.Immunologic research · 2024Article
- Easy recognition and high autoimmune hepatitis specificity of smooth muscle antibodies giving an actin microfilament immunofluorescent pattern on embryonal vascular smooth muscle cells.Clinical and experimental immunology · 2024Article
- Evaluation of a Fully Automated Antinuclear Antibody Indirect Immunofluorescence Assay in Routine Use.Frontiers in immunology · 2020Article
- Antinuclear antibodies detection: A comparative study between automated recognition and conventional visual interpretation.Journal of clinical laboratory analysis · 2019Article
- Performance analysis of automated evaluation of antinuclear antibody indirect immunofluorescent tests in a routine setting.Auto- immunity highlights · 2018Article
- Automated Processing and Evaluation of Anti-Nuclear Antibody Indirect Immunofluorescence Testing.Frontiers in immunology · 2018Article
- Genotypic and Phenotypic Characteristics Associated with Biofilm Formation by Human Clinical Escherichia coli Isolates of Different Pathotypes.Applied and environmental microbiology · 2017Article
- Recent Approaches To Optimize Laboratory Assessment of Antinuclear Antibodies.Clinical and vaccine immunology : CVI · 2017Review
- Next-Generation Autoantibody Testing by Combination of Screening and Confirmation-the CytoBead® Technology.Clinical reviews in allergy & immunology · 2017Review
- Anti-nuclear autoantibodies in the general German population: prevalence and lack of association with selected cardiovascular and metabolic disorders-findings of a multicenter population-based study.Arthritis research & therapy · 2017Article
- The burden of the variability introduced by the HEp-2 assay kit and the CAD system in ANA indirect immunofluorescence test.Immunologic research · 2017Article
- ANA IIF Automation: Moving towards Harmonization? Results of a Multicenter Study.Journal of immunology research · 2017Article
- Automated evaluation of ANA under real-life conditions.RMD open · 2017Article
- The Reliability of a Novel Automated System for ANA Immunofluorescence Analysis in Daily Clinical Practice.International journal of rheumatology · 2016Article
- Clinical performance evaluation of a novel, automated chemiluminescent immunoassay, QUANTA Flash CTD Screen Plus.Immunologic research · 2015Article
- A fully automated IIF system for the detection of antinuclear antibodies and antineutrophil cytoplasmic antibodies.Immunologic research · 2015Article
- Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology.Journal of immunology research · 2015Article
- [Rheumatoid factor or antinuclear antibodies as incidental finding].Der Internist · 2014Article
- In-vitro and in-vivo imaging of prostate tumor using NaYF4: Yb, Er up-converting nanoparticles.Pathology oncology research : POR · 2014Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionAnalysis of autoantibodies (AAB) by indirect immunofluorescence (IIF) is a basic tool for the serological diagnosis of systemic rheumatic disorders. Automation of autoantibody IIF reading including pattern recognition may improve intra- and inter-laboratory variability and meet the demand for cost-effective assessment of large numbers of samples. Comparing automated and visual interpretation, the usefulness for routine laboratory diagnostics was investigated.
methodsAutoantibody detection by IIF on human epithelial-2 (HEp-2) cells was conducted in a total of 1222 consecutive sera of patients with suspected systemic rheumatic diseases from a university routine laboratory (n = 924) and a private referral laboratory (n = 298). IIF results from routine diagnostics were compared with a novel automated interpretation system.
resultsBoth diagnostic procedures showed a very good agreement in detecting AAB (kappa = 0.828) and differentiating respective immunofluorescence patterns. Only 98 (8.0%) of 1222 sera demonstrated discrepant results in the differentiation of positive from negative samples. The contingency coefficients of chi-square statistics were 0.646 for the university laboratory cohort with an agreement of 93.0% and 0.695 for the private laboratory cohort with an agreement of 90.6%, P < 0.0001, respectively. Comparing immunofluorescence patterns, 111 (15.3%) sera yielded differing results.
conclusionsAutomated assessment of AAB by IIF on HEp-2 cells using an automated interpretation system is a reliable and robust method for positive/negative differentiation. Employing novel mathematical algorithms, automated interpretation provides reproducible detection of specific immunofluorescence patterns on HEp-2 cells. Automated interpretation can reduce drawbacks of IIF for AAB detection in routine diagnostics providing more reliable data for clinicians.
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