ArticleGenesis (New York, N.Y. : 2000)2010
Ehd4 is required to attain normal prepubertal testis size but dispensable for fertility in male mice.
Article in Genesis (New York, N.Y. : 2000), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 25 citations in OpenAlex.
- A Quarter Century of EHD Protein Research: From Endosomal Recycling to Ciliopathies.Traffic (Copenhagen, Denmark) · 2026Review
- Genome-wide association study exploring the genetic architecture of eggshell speckles in laying hens.BMC genomics · 2023Article
- Combined analysis of mRNA-miRNA from testis tissue in Tibetan sheep with different FecB genotypes.Open life sciences · 2023Article
- Gut Microbiota Dysbiosis Induced by Decreasing Endogenous Melatonin Mediates the Pathogenesis of Alzheimer's Disease and Obesity.Frontiers in immunology · 2022Article
- ARP-T1-associated Bazex-Dupré-Christol syndrome is an inherited basal cell cancer with ciliary defects characteristic of ciliopathies.Communications biology · 2021Article
- Article
- Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1.Scientific reports · 2018Article
- Role of the EHD Family of Endocytic Recycling Regulators for TCR Recycling and T Cell Function.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- EHD4 is a novel regulator of urinary water homeostasis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- CSF-1 receptor signalling is governed by pre-requisite EHD1 mediated receptor display on the macrophage cell surface.Cellular signalling · 2016Article
- The Magea gene cluster regulates male germ cell apoptosis without affecting the fertility in mice.Scientific reports · 2016Article
- Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development.Scientific reports · 2016Article
- The endocytic recycling regulatory protein EHD1 Is required for ocular lens development.Developmental biology · 2015Article
- Effects of Korean Red Ginseng extract on busulfan-induced dysfunction of the male reproductive system.Journal of ginseng research · 2015Article
- EHD1 mediates vesicle trafficking required for normal muscle growth and transverse tubule development.Developmental biology · 2014Article
- Eps homology domain endosomal transport proteins differentially localize to the neuromuscular junction.Skeletal muscle · 2012Article
- Rabs and EHDs: alternate modes for traffic control.Bioscience reports · 2012Review
- Renal thrombotic microangiopathy in mice with combined deletion of endocytic recycling regulators EHD3 and EHD4.PloS one · 2011Article
- EHD proteins: key conductors of endocytic transport.Trends in cell biology · 2011Review
- Ferlin proteins in myoblast fusion and muscle growth.Current topics in developmental biology · 2011Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
The four highly homologous members of the C-terminal EH domain-containing (EHD) protein family (EHD1-4) regulate endocytic recycling. To delineate the role of EHD4 in normal physiology and development, mice with a conditional knockout of the Ehd4 gene were generated. PCR of genomic DNA and Western blotting of organ lysates from Ehd4(-/-) mice confirmed EHD4 deletion. Ehd4(-/-) mice were viable and born at expected Mendelian ratios; however, males showed a 50% reduction in testis weight, obvious from postnatal day 31. An early (Day 10) increase in germ cell proliferation and apoptosis and a later increase in apoptosis (Day 31) were seen in the Ehd4(-/-) testis. Other defects included a progressive reduction in seminiferous tubule diameter, dysregulation of seminiferous epithelium, and head abnormalities in elongated spermatids. As a consequence, lower sperm counts and reduced fertility were observed in Ehd4(-/-) males. Interestingly, EHD protein expression was seen to be temporally regulated in the testis and EHD4 levels peaked between days 10 and 15. In the adult testis, EHD4 was highly expressed in primary spermatocytes and EHD4 deletion altered the levels of other EHD proteins in an age-dependent manner. We conclude that high levels of EHD1 in the adult Ehd4(-/-) testis functionally compensate for lack of EHD4 and prevents the development of severe fertility defects. Our results suggest a role for EHD4 in the proper development of postmitotic and postmeiotic germ cells and implicate EHD protein-mediated endocytic recycling as an important process in germ cell development and testis function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.