ArticleHypertension (Dallas, Tex. : 1979)2010
Female adult mouse cardiomyocytes are protected against oxidative stress.
Article in Hypertension (Dallas, Tex. : 1979), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- Estrogen receptor α gene PvuII polymorphism and coronary artery disease: a meta-analysis of 21 studies.Journal of Zhejiang University. Science. B · 2014Pooled it
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- Gender disparities in outcomes of cardiogenic shock secondary to Takotsubo cardiomyopathy.American heart journal plus : cardiology research and practice · 2024Article
- Reduced cardiac antioxidant defenses mediate increased susceptibility to workload-induced myocardial injury in males with genetic cardiomyopathy.Journal of molecular and cellular cardiology · 2024Article
- Lack of STAT1 co-operative DNA binding protects against adverse cardiac remodelling in acute myocardial infarction.Frontiers in cardiovascular medicine · 2023Article
- Mitochondrial respiration in C57BL/6 substrains varies in response to myocardial infarction.Journal of bioenergetics and biomembranes · 2021Article
- The Determining Role of Mitochondrial Reactive Oxygen Species Generation and Monoamine Oxidase Activity in Doxorubicin-Induced Cardiotoxicity.Antioxidants & redox signaling · 2021Article
- Amylin deposition activates HIF1α and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates.Communications biology · 2021Article
- [A retrospective mortality analysis of natural deaths of the 65+ generation based on postmortem autopsies performed at the Institute of Legal Medicine in Frankfurt am Main during two periods].Rechtsmedizin (Berlin, Germany) · 2021Article
- Ageing, sex, and cardioprotection.British journal of pharmacology · 2020Review
- Lower Post Myocardial Infarction Mortality Among Women Treated at Veterans Affairs Hospitals Compared to Men.The American journal of the medical sciences · 2020Article
- Selected sex related differences in pathophysiology of cardiovascular system.Physiological research · 2020Review
- The Influence of Sex on Cardiac Physiology and Cardiovascular Diseases.Journal of cardiovascular translational research · 2020Review
- The Impact of Skeletal Muscle ERα on Mitochondrial Function and Metabolic Health.Endocrinology · 2020Review
- Sex Hormone Regulation of Proteins Modulating Mitochondrial Metabolism, Dynamics and Inter-Organellar Cross Talk in Cardiovascular Disease.Frontiers in cell and developmental biology · 2020Review
- Necrosis Contributes to the Development of Hypertension in Male, but Not Female, Spontaneously Hypertensive Rats.Hypertension (Dallas, Tex. : 1979) · 2019Article
- Sex differences in anthracycline-induced cardiotoxicity: the benefits of estrogens.Heart failure reviews · 2019Review
- The impact of sex and myocardial ischemic preconditioning on immunohistochemical markers of acute myocardial infarction.International journal of legal medicine · 2019Article
- Sex differences in distribution of cannabinoid receptors (CB1 and CB2), S100A6 and CacyBP/SIP in human ageing hearts.Biology of sex differences · 2018Article
- The impact of ERα action on muscle metabolism and insulin sensitivity - Strong enough for a man, made for a woman.Molecular metabolism · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
Premenopausal women have less cardiovascular disease and lower cardiovascular morbidity and mortality than men the same age. Our previous studies showed that female mice have lower mortality and better preserved cardiac function after myocardial infarction. However, the precise cellular and molecular mechanisms responsible for such a sex difference are not well established. Using cultured adult mouse cardiomyocytes, we tested the hypothesis that the survival advantage of females stems from activated estrogen receptors and Akt survival signaling pathways. Adult mouse cardiomyocytes were isolated from male and female C57BL/6J mice and treated with hydrogen peroxide (100 micromol/L) for 30 minutes. Cell survival was indicated by rod ratio (rod shaped cells:total cells), cell death by lactate dehydrogenase release, and positive staining of annexin-V (a marker for apoptosis) and propidium iodide (a marker for necrosis). In response to hydrogen peroxide(,) female adult mouse cardiomyocytes exhibited a higher rod ratio, lower lactate dehydrogenase release, and fewer Annexin-V-positive and propidium iodide-positive cells compared with males. Phospho-Akt was greater in females both at baseline and after hydrogen peroxide stimulation. The downstream molecule of Akt, phosphor-GSK-3beta (inactivation), was also higher, whereas caspase 3 activity was lower in females in response to hydrogen peroxide. Bcl-2 did not differ between sexes. Estrogen receptor-alpha was the dominant isoform in females, whereas estrogen receptor-beta was low but similar in both sexes. Our findings demonstrate that female adult mouse cardiomyocytes have a greater survival advantage when challenged with oxidative stress-induced cell death. This may be attributable to activation of Akt and inhibition of GSK-3beta and caspase 3 through an estrogen receptor-alpha-mediated mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.