ArticleProceedings of the National Academy of Sciences of the United States of America2010
Characterization of somatic cell nuclear reprogramming by oocytes in which a linker histone is required for pluripotency gene reactivation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 106 citations in OpenAlex.
- Epigenetics in development, disease, and therapy: from mechanisms to clinical interventions.Science China. Life sciences · 2026Review
- Reversibility, regulation, and the community of development: the legacy of Sir John B. Gurdon.Nature communications · 2025Review
- Heterologous expression of bovine histone H1foo into porcine fibroblasts alters the transcriptome profile but not embryo development following nuclear transfer.Journal of assisted reproduction and genetics · 2025Article
- Histone variants: key regulators of inflammation in cell dedifferentiation and transdifferentiation.Frontiers in immunology · 2025Review
- Chromatin accessibility dynamics during cell fate reprogramming.EMBO reports · 2021Review
- The embryonic linker histone dBigH1 alters the functional state of active chromatin.Nucleic acids research · 2020Article
- Various nuclear reprogramming systems using egg and oocyte materials.The Journal of reproduction and development · 2019Review
- Binding of HMGN proteins to cell specific enhancers stabilizes cell identity.Nature communications · 2018Article
- Chromatin remodeling in Drosophila preblastodermic embryo extract.Scientific reports · 2018Article
- Dedifferentiation of human uterine polyp stem cells into embryo-like cells during inducing pluripotency.International journal of biological sciences · 2018Article
- Nuclear Actin in Development and Transcriptional Reprogramming.Frontiers in genetics · 2017Review
- RE1-silencing Transcription Factor (REST) Is Required for Nuclear Reprogramming by Inhibiting Transforming Growth Factor β Signaling Pathway.The Journal of biological chemistry · 2016Article
- Cycling through developmental decisions: how cell cycle dynamics control pluripotency, differentiation and reprogramming.Development (Cambridge, England) · 2016Review
- H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells.Stem cell reports · 2016Article
- Germline-specific H1 variants: the "sexy" linker histones.Chromosoma · 2016Review
- Role of H1 linker histones in mammalian development and stem cell differentiation.Biochimica et biophysica acta · 2016Review
- Chaperone-mediated chromatin assembly and transcriptional regulation in Xenopus laevis.The International journal of developmental biology · 2016Review
- The H1 linker histones: multifunctional proteins beyond the nucleosomal core particle.EMBO reports · 2015Review
- A Specialized Histone H1 Variant Is Required for Adaptive Responses to Complex Abiotic Stress and Related DNA Methylation in Arabidopsis.Plant physiology · 2015Article
- The Egg and the Nucleus: A Battle for Supremacy.Rambam Maimonides medical journal · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
When transplanted into Xenopus oocytes, the nuclei of mammalian somatic cells are reprogrammed to express stem cell genes such as Oct4, Nanog, and Sox2. We now describe an experimental system in which the pluripotency genes Sox2 and Oct4 are repressed in retinoic acid-treated ES cells but are reprogrammed up to 100% within 24 h by injection of nuclei into the germinal vesicle (GV) of growing Xenopus oocytes. The isolation of GVs in nonaqueous medium allows the reprogramming of individual injected nuclei to be seen in real time. Analysis using fluorescence recovery after photobleaching shows that nuclear transfer is associated with an increase in linker histone mobility. A simultaneous loss of somatic H1 linker histone and incorporation of the oocyte-specific linker histone B4 precede transcriptional reprogramming. The loss of H1 is not required for gene reprogramming. We demonstrate both by antibody injection experiments and by dominant negative interference that the incorporation of B4 linker histone is required for pluripotency gene reactivation during nuclear reprogramming. We suggest that the binding of oocyte-specific B4 linker histone to chromatin is a key primary event in the reprogramming of somatic nuclei transplanted to amphibian oocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.