ArticleThe Journal of physiology2010
Transient release kinetics of rod bipolar cells revealed by capacitance measurement of exocytosis from axon terminals in rat retinal slices.
Article in The Journal of physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- High-Pressure Freezing EM Tomography of Entire Ribbon Synapses in the Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Capacitance Measurements of Exocytosis From AII Amacrine Cells in Retinal Slices.Bio-protocol · 2025Article
- Functional maturation of the rod bipolar to AII-amacrine cell ribbon synapse in the mouse retina.Cell reports · 2023Article
- Trophoblast glycoprotein is required for efficient synaptic vesicle exocytosis from retinal rod bipolar cells.Frontiers in cellular neuroscience · 2023Article
- Dendritic Morphology of an Inhibitory Retinal Interneuron Enables Simultaneous Local and Global Synaptic Integration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Article
- Capacitance measurement of dendritic exocytosis in an electrically coupled inhibitory retinal interneuron: an experimental and computational study.Physiological reports · 2019Article
- Electric stimulus duration alters network-mediated responses depending on retinal ganglion cell type.Journal of neural engineering · 2018Article
- Contributions of Rod and Cone Pathways to Retinal Direction Selectivity Through Development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Functional Circuitry of the Retina.Annual review of vision science · 2015Article
- Synaptic Vesicle Exocytosis at the Dendritic Lobules of an Inhibitory Interneuron in the Mammalian Retina.Neuron · 2015Article
- Rapid kinetics of endocytosis at rod photoreceptor synapses depends upon endocytic load and calcium.Visual neuroscience · 2014Article
- Global Ca2+ signaling drives ribbon-independent synaptic transmission at rod bipolar cell synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Regulation of presynaptic calcium in a mammalian synaptic terminal.Journal of neurophysiology · 2012Article
- Carbonic anhydrase-related protein VIII is expressed in rod bipolar cells and alters signaling at the rod bipolar to AII-amacrine cell synapse in the mammalian retina.The European journal of neuroscience · 2011Article
- Calcium channel dynamics limit synaptic release in response to prosthetic stimulation with sinusoidal waveforms.Journal of neural engineering · 2011Article
- A synaptic mechanism for retinal adaptation to luminance and contrast.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Synaptic release at mammalian bipolar cell terminals.Visual neuroscience · 2011Review
- Extending the realm of membrane capacitance measurements to nerve terminals with complex morphologies.The Journal of physiology · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Presynaptic transmitter release has mostly been studied through measurements of postsynaptic responses, but a few synapses offer direct access to the presynaptic terminal, thereby allowing capacitance measurements of exocytosis. For mammalian rod bipolar cells, synaptic transmission has been investigated in great detail by recording postsynaptic currents in AII amacrine cells. Presynaptic measurements of the dynamics of vesicular cycling have so far been limited to isolated rod bipolar cells in dissociated preparations. Here, we first used computer simulations of compartmental models of morphologically reconstructed rod bipolar cells to adapt the 'Sine + DC' technique for capacitance measurements of exocytosis at axon terminals of intact rod bipolar cells in retinal slices. In subsequent physiological recordings, voltage pulses that triggered presynaptic Ca(2+) influx evoked capacitance increases that were proportional to the pulse duration. With pulse durations 100 ms, the increase saturated at 10 fF, corresponding to the size of a readily releasable pool of vesicles. Pulse durations 400 ms evoked additional capacitance increases, probably reflecting recruitment from additional pools of vesicles. By using Ca(2+) tail current stimuli, we separated Ca(2+) influx from Ca(2+) channel activation kinetics, allowing us to estimate the intrinsic release kinetics of the readily releasable pool, yielding a time constant of 1.1 ms and a maximum release rate of 2-3 vesicles (release site)(1) ms(1). Following exocytosis, we observed endocytosis with time constants ranging from 0.7 to 17 s. Under physiological conditions, it is likely that release will be transient, with the kinetics limited by the activation kinetics of the voltage-gated Ca(2+) channels.
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