ArticleNucleic acids research2010
Cloning whole bacterial genomes in yeast.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 166 citations in OpenAlex.
- Capsular polysaccharide of Mycoplasma mycoides subsp. capri contributes to phenotypic diversity promoting distinctive immune responses.PLoS pathogens · 2025Article
- The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025Review
- Minimal Bacterial Cell JCVI-syn3B as a Chassis to Investigate Interactions between Bacteria and Mammalian Cells.ACS synthetic biology · 2024Article
- An Expanded Genetic Toolbox to Accelerate the Creation ofACS synthetic biology · 2024Article
- Modular Assembly of Synthetic Secondary Chromosomes.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Cloning and sequencing analysis of wholeFrontiers in microbiology · 2024Article
- Review
- Combining Fusion of Cells with CRISPR-Cas9 Editing for the Cloning of Large DNA Fragments or Complete Bacterial Genomes in Yeast.ACS synthetic biology · 2023Article
- Gene editing tools for mycoplasmas: references and future directions for efficient genome manipulation.Frontiers in microbiology · 2023Review
- Cross-species microbial genome transfer: a Review.Frontiers in bioengineering and biotechnology · 2023Review
- Genome Engineering of the Fast-GrowingACS synthetic biology · 2022Article
- Conjugation-Based Genome Engineering inACS synthetic biology · 2022Article
- Synthetic Genomics From a Yeast Perspective.Frontiers in bioengineering and biotechnology · 2022Review
- Seeding the idea of encapsulating a representative synthetic metagenome in a single yeast cell.Nature communications · 2021Article
- A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- Building genomes to understand biology.Nature communications · 2020Review
- Integrative characterization of the near-minimal bacterium Mesoplasma florum.Molecular systems biology · 2020Article
- Budding yeast as a factory to engineer partial and complete microbial genomes.Current opinion in systems biology · 2020Review
- Genetic Engineering and Synthetic Genomics in Yeast to Understand Life and Boost Biotechnology.Bioengineering (Basel, Switzerland) · 2020Review
- Cloning ofBiology · 2020Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
18 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional characterization. Here we report the cloning of whole bacterial genomes in the yeast Saccharomyces cerevisiae as single-DNA molecules. We cloned the genomes of Mycoplasma genitalium (0.6 Mb), M. pneumoniae (0.8 Mb) and M. mycoides subspecies capri (1.1 Mb) as yeast circular centromeric plasmids. These genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.