ArticleNucleic acids research2010
Tuning and controlling gene expression noise in synthetic gene networks.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
80 citing papers in PubMed, 1 synthesis or guideline pooled it, 148 citations in OpenAlex.
- Pooled it
- SUPER: Upcycling Genetic Parts for Precise Gene Expression Control, Leakage Minimization, and Genetic Circuit Stability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The switch-liker's guide to plant synthetic gene circuits.The Plant journal : for cell and molecular biology · 2025Review
- Transcription Attenuation in Synthetic Promoters in Nonoverlapping Tandem Formation.Biochemistry · 2024Article
- Contributions of transcriptional noise to leukaemia evolution: KAT2A as a case-study.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Designing artificial synthetic promoters for accurate, smart, and versatile gene expression in plants.Plant communications · 2023Review
- Transcription factor fluctuations underlie cell-to-cell variability in a signaling pathway response.Genetics · 2023Article
- Tuning the Transcriptional Activity of the CaMV 35S Promoter in Plants by Single-Nucleotide Changes in the TATA Box.ACS synthetic biology · 2023Article
- Double-edged role of resource competition in gene expression noise and control.Advanced genetics (Hoboken, N.J.) · 2022Article
- Principles for the design of multicellular engineered living systems.APL bioengineering · 2022Article
- Dynamic modulation of enhancer responsiveness by core promoter elements in living Drosophila embryos.Nucleic acids research · 2022Article
- Independent control of mean and noise by convolution of gene expression distributions.Nature communications · 2021Article
- Reduction in gene expression noise by targeted increase in accessibility at gene loci.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The switch of DNA states filtering the extrinsic noise in the system of frequency modulation.Scientific reports · 2021Article
- 3Genome biology · 2021Article
- Orthogonal control of mean and variability of endogenous genes in a human cell line.Nature communications · 2021Article
- Orthogonal tuning of gene expression noise using CRISPR-Cas.Nucleic acids research · 2020Article
- Making Sense of Computational Psychiatry.The international journal of neuropsychopharmacology · 2020Review
- Allele-specific single-cell RNA sequencing reveals different architectures of intrinsic and extrinsic gene expression noises.Nucleic acids research · 2020Article
- Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Synthetic gene networks can be used to control gene expression and cellular phenotypes in a variety of applications. In many instances, however, such networks can behave unreliably due to gene expression noise. Accordingly, there is a need to develop systematic means to tune gene expression noise, so that it can be suppressed in some cases and harnessed in others, e.g. in cellular differentiation to create population-wide heterogeneity. Here, we present a method for controlling noise in synthetic eukaryotic gene expression systems, utilizing reduction of noise levels by TATA box mutations and noise propagation in transcriptional cascades. Specifically, we introduce TATA box mutations into promoters driving TetR expression and show that these mutations can be used to effectively tune the noise of a target gene while decoupling it from the mean, with negligible effects on the dynamic range and basal expression. We apply mathematical and computational modeling to explain the experimentally observed effects of TATA box mutations. This work, which highlights some important aspects of noise propagation in gene regulatory cascades, has practical implications for implementing gene expression control in synthetic gene networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.