ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2010
Diagnostic assays for active infection with human herpesvirus 6 (HHV-6).
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 88 citations in OpenAlex.
- Denominator and Workflow Dependence of Age-Specific Viral Detection in Cerebrospinal Fluid.Pathogens (Basel, Switzerland) · 2026Article
- Performance evaluation of the SMG HHV-6 Q Real-Time PCR Kit for quantitative detection and differentiation of human herpesvirus 6A and 6B.Microbiology spectrum · 2024Article
- Inherited Chromosomally Integrated Human Herpesvirus 6: Laboratory and Clinical Features.Microorganisms · 2023Article
- A Rare Case of HHV-6 Encephalitis in an Immunocompetent Host: Case Report and Literature Review.Cureus · 2022Article
- Human Herpesvirus 6A Is a Risk Factor for Multiple Sclerosis.Frontiers in immunology · 2022Review
- Human Herpesvirus 6 (HHV-6) Encephalitis in a Non-Transplant Patient With Polymyositis.Cureus · 2021Article
- Multicenter prospective surveillance study of viral agents causing meningoencephalitis.Scientific reports · 2021Observational
- Human herpesvirus 6 meningitis in an adult.Proceedings (Baylor University. Medical Center) · 2020Article
- RNA Sequencing of theJournal of virology · 2019Article
- Review
- A Celebration of the Life and Work of Caroline Breese Hall, MD.Journal of the Pediatric Infectious Diseases Society · 2016Article
- Article
- Type specific Real time PCR for detection of human herpes virus 6 in schizophrenia and bipolar patients: a case control study.BMC psychiatry · 2015Article
- Laboratory and clinical aspects of human herpesvirus 6 infections.Clinical microbiology reviews · 2015Review
- Past, present, and future perspectives on the diagnosis of Roseolovirus infections.Current opinion in virology · 2014Review
- Identification of chromosomally integrated human herpesvirus 6 by droplet digital PCR.Clinical chemistry · 2014Article
- Detection of spliced mRNA from human bocavirus 1 in clinical samples from children with respiratory tract infections.Emerging infectious diseases · 2013Article
- Article
- Chromosomally integrated human herpesvirus 6: questions and answers.Reviews in medical virology · 2012Review
- Molecular diagnosis and management of viral infections in hematopoietic stem cell transplant recipients.Molecular diagnosis & therapy · 2012Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
backgroundHuman herpesvirus 6 (HHV-6) causes ubiquitous infection in early childhood with lifelong latency or persistence. Reactivation of HHV-6 has been associated with multiple diseases including encephalitis. Chromosomal integration of HHV-6 also occurs. Previous studies have suggested that the detection of HHV-6 DNA in plasma is an accurate marker of active viral replication.
objectiveWe sought to determine whether PCR assays on plasma could correctly differentiate between primary HHV-6 infection, chromosomal integration of HHV-6 and latent HHV-6 infection. STUDY
designWe performed qualitative PCR, real-time quantitative PCR (RQ-PCR), and reverse-transcriptase PCR (RT-PCR) assays on samples of peripheral and cord blood mononuclear cells, as well as plasma, from groups of subjects with well defined HHV-6 infection, including subjects with chromosomally integrated HHV-6. RESULTS AND
conclusionsThe detection of HHV-6 DNA in plasma was 92% sensitive compared to viral isolation for the identification of primary infection with HHV-6. All plasma samples from infants with chromosomally integrated HHV-6 had HHV-6 DNA detectable in plasma while only 5.6% were positive by RT-PCR. The specificity of plasma PCR for active replication of HHV-6 was 84% compared to viral culture while the specificity of RT-PCR was 98%. Our results demonstrate that qualitative or quantitative PCR of plasma is insufficient to distinguish between active viral replication and chromosomal integration with HHV-6. We found a higher specificity of RT-PCR performed on PBMC samples compared to PCR or RQ-PCR performed on plasma when evaluating samples for active HHV-6 replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.