ArticleBMC molecular biology2010
Identification of regulatory elements flanking human XIST reveals species differences.
Article in BMC molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- The compleX balancing act of controlling X-chromosome dosage and how it impacts mammalian germline development.The Biochemical journal · 2023Article
- A NovelMolecular and cellular biology · 2021Article
- DNA hypermethylation and differential gene expression associated with Klinefelter syndrome.Scientific reports · 2018Article
- X-chromosome inactivation and escape.Journal of genetics · 2015Review
- Uncoupling of X-linked gene silencing from XIST binding by DICER1 and chromatin modulation on human inactive X chromosome.Chromosoma · 2015Article
- Differentially methylated CpG island within human XIST mediates alternative P2 transcription and YY1 binding.BMC genetics · 2014Article
- X chromosome regulation: diverse patterns in development, tissues and disease.Nature reviews. Genetics · 2014Review
- Evolutionary diversity and developmental regulation of X-chromosome inactivation.Human genetics · 2011Review
- The single active X in human cells: evolutionary tinkering personified.Human genetics · 2011Review
- Role of ATRX in chromatin structure and function: implications for chromosome instability and human disease.Reproduction (Cambridge, England) · 2011Review
- XCI in preimplantation mouse and human embryos: first there is remodelling….Human genetics · 2011Review
- X-chromosome inactivation: molecular mechanisms from the human perspective.Human genetics · 2011Review
- Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development.Nature · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe transcriptional silencing of one X chromosome in eutherians requires transcription of the long non-coding RNA gene, XIST. Many regulatory elements have been identified downstream of the mouse Xist gene, including the antisense Tsix gene. However, these elements do not show sequence conservation with humans, and the human TSIX gene shows critical differences from the mouse. Thus we have undertaken an unbiased identification of regulatory elements both downstream and upstream of the human XIST gene using DNase I hypersensitivity mapping.
resultsDownstream of XIST a single DNase I hypersensitive site was identified in a mouse undifferentiated ES cell line containing an integration of the human XIC region. This site was not observed in somatic cells. Upstream of XIST, the distance to the flanking JPX gene is expanded in humans relative to mice, and we observe a hypersensitive site 65 kb upstream of XIST, in addition to hypersensitive sites near the XIST promoter. This -65 region has bi-directional promoter activity and shows sequence conservation in non-rodent eutheria.
conclusionsThe lack of regulatory elements corresponding to human TSIX lends further support to the argument that TSIX is not a regulator of XIST in humans. The upstream hypersensitive sites we identify show sequence conservation with other eutheria, but not with mice. Therefore the regulation of XIST seems to be different between mice and man, and regulatory sequences upstream of XIST may be important regulators of XIST in non-rodent eutheria instead of Tsix which is critical for Xist regulation in rodents.
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