ArticleBMC genomics2010
Transcriptome profiling of a Sinorhizobium meliloti fadD mutant reveals the role of rhizobactin 1021 biosynthesis and regulation genes in the control of swarming.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- Rhizobial motility preference in root colonization of Medicago truncatula.The New phytologist · 2026Article
- Identification of a Sinorhizobium meliloti YbgC-like thioesterase that contributes to the production of the infochemical 2-tridecanone.The Biochemical journal · 2025Article
- Siderophores and competition for iron govern myxobacterial predation dynamics.The ISME journal · 2024Article
- Article
- Surface Motility Regulation ofInternational journal of molecular sciences · 2022Article
- Comparative Genomics across Three Ensifer Species Using a New Complete Genome Sequence of the Medicago SymbiontMicroorganisms · 2021Article
- ExoS/ChvI Two-Component Signal-Transduction System Activated in the Absence of Bacterial Phosphatidylcholine.Frontiers in plant science · 2021Article
- A Framework for the Selection of Plant Growth-Promoting Rhizobacteria Based on Bacterial Competence Mechanisms.Applied and environmental microbiology · 2020Article
- Role ofMicroorganisms · 2020Article
- Article
- Article
- Fatty acid-releasing activities in Sinorhizobium meliloti include unusual diacylglycerol lipase.Environmental microbiology · 2015Article
- Biofilm formation assessment in Sinorhizobium meliloti reveals interlinked control with surface motility.BMC microbiology · 2015Article
- Genomic characterization of Sinorhizobium meliloti AK21, a wild isolate from the Aral Sea Region.SpringerPlus · 2015Article
- RNase E affects the expression of the acyl-homoserine lactone synthase gene sinI in Sinorhizobium meliloti.Journal of bacteriology · 2014Article
- ExpR coordinates the expression of symbiotically important, bundle-forming Flp pili with quorum sensing in Sinorhizobium meliloti.Applied and environmental microbiology · 2014Article
- A chimeric siderophore halts swarming Vibrio.Angewandte Chemie (International ed. in English) · 2014Article
- ExpR is not required for swarming but promotes sliding in Sinorhizobium meliloti.Journal of bacteriology · 2012Article
- FadD is required for utilization of endogenous fatty acids released from membrane lipids.Journal of bacteriology · 2011Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSwarming is a multicellular phenomenom characterized by the coordinated and rapid movement of bacteria across semisolid surfaces. In Sinorhizobium meliloti this type of motility has been described in a fadD mutant. To gain insights into the mechanisms underlying the process of swarming in rhizobia, we compared the transcriptome of a S. meliloti fadD mutant grown under swarming inducing conditions (semisolid medium) to those of cells grown under non-swarming conditions (broth and solid medium).
resultsMore than a thousand genes were identified as differentially expressed in response to growth on agar surfaces including genes for several metabolic activities, iron uptake, chemotaxis, motility and stress-related genes. Under swarming-specific conditions, the most remarkable response was the up-regulation of iron-related genes. We demonstrate that the pSymA plasmid and specifically genes required for the biosynthesis of the siderophore rhizobactin 1021 are essential for swarming of a S. meliloti wild-type strain but not in a fadD mutant. Moreover, high iron conditions inhibit swarming of the wild-type strain but not in mutants lacking either the iron limitation response regulator RirA or FadD.
conclusionsThe present work represents the first transcriptomic study of rhizobium growth on surfaces including swarming inducing conditions. The results have revealed major changes in the physiology of S. meliloti cells grown on a surface relative to liquid cultures. Moreover, analysis of genes responding to swarming inducing conditions led to the demonstration that iron and genes involved in rhizobactin 1021 synthesis play a role in the surface motility shown by S. meliloti which can be circumvented in a fadD mutant. This work opens a way to the identification of new traits and regulatory networks involved in swarming by rhizobia.
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