ArticleJournal of biomedical optics
Calcium imaging of inner ear hair cells within the cochlear epithelium of mice using two-photon microscopy.
Article in Journal of biomedical optics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Altered Outer Hair Cell Mitochondrial and Subsurface Cisternae Connectomics Are Candidate Mechanisms for Hearing Loss in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Imaging Bioluminescent Exogenous Stem Cells in the Intact Guinea Pig Cochlea.Anatomical record (Hoboken, N.J. : 2007) · 2020Article
- Osmotic stabilization prevents cochlear synaptopathy after blast trauma.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Activity-dependent regulation of prestin expression in mouse outer hair cells.Journal of neurophysiology · 2015Article
- Prestin regulation and function in residual outer hair cells after noise-induced hearing loss.PloS one · 2013Article
- Article
- Monitoring intracellular calcium ion dynamics in hair cell populations with Fluo-4 AM.PloS one · 2012Article
- Biophysical mechanisms underlying outer hair cell loss associated with a shortened tectorial membrane.Journal of the Association for Research in Otolaryngology : JARO · 2011Article
- A series of carbazole cationic compounds with large two-photon absorption cross sections for imaging mitochondria in living cells with two-photon fluorescence microscopy.Journal of fluorescence · 2011Article
- Deficient forward transduction and enhanced reverse transduction in the alpha tectorin C1509G human hearing loss mutation.Disease models & mechanismsArticle
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Authors and funding
4 authors.
Funding
Abstract
Mice are an excellent model for studying mammalian hearing and transgenic mouse models of human hearing, loss are commonly available. However, the mouse cochlea is substantially smaller than other animal models routinely used to study cochlear physiology. This makes study of their hair cells difficult. We develop a novel methodology to optically image calcium within living hair cells left undisturbed within the excised mouse cochlea. Fresh cochleae are harvested, left intact within their otic capsule bone, and fixed in a recording chamber. The bone overlying the cochlear epithelium is opened and Reissner's membrane is incised. A fluorescent calcium indicator is applied to the preparation. A custom-built upright two-photon microscope was used to image the preparation using 3-D scanning. We are able to image about one third of a cochlear turn simultaneously, in either the apical or basal regions. Within one hour of animal sacrifice, we find that outer hair cells demonstrate increased fluorescence compared with surrounding supporting cells. This methodology is then used to visualize hair cell calcium changes during mechanotransduction over a region of the epithelium. Because the epithelium is left within the cochlea, dissection trauma is minimized and artifactual changes in hair cell physiology are expected to be reduced.
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Registered trials
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