ArticleJournal of biomedical optics
Use of optical tweezers to probe epithelial mechanosensation.
Article in Journal of biomedical optics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Altered ciliary morphology reduces mechanosensation in a cystic kidney model as indicated by a mathematical model.Scientific reports · 2026Article
- Cilia function as calcium-mediated mechanosensors that instruct left-right asymmetry.Science (New York, N.Y.) · 2023Article
- Substrate Stiffness Mediates Formation of Novel Cytoskeletal Structures in Fibroblasts during Cell-Microspheres Interaction.International journal of molecular sciences · 2021Article
- Primary cilia have a length-dependent persistence length.Biomechanics and modeling in mechanobiology · 2020Article
- Microtubules regulate cardiomyocyte transversal Young's modulus.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- HIF Stabilization Weakens Primary Cilia.PloS one · 2016Article
- Article
- Discerning the role of mechanosensors in regulating proximal tubule function.American journal of physiology. Renal physiology · 2016Review
- Patterning of wound-induced intercellular Ca(2+) flashes in a developing epithelium.Physical biology · 2015Article
- Mechanosensory calcium signaling.Advances in experimental medicine and biology · 2012Review
- An Integrative Review of Mechanotransduction in Endothelial, Epithelial (Renal) and Dendritic Cells (Osteocytes).Cellular and molecular bioengineering · 2011Article
- More than a feeling: discovering, understanding, and influencing mechanosensing pathways.Current opinion in biotechnology · 2011Article
- Article
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Authors and funding
1 author at 1 institution in 1 country.
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Abstract
Cellular mechanosensation mechanisms have been implicated in a variety of disease states. Specifically in renal tubules, the primary cilium and associated mechanosensitive ion channels are hypothesized to play a role in water and salt homeostasis, with relevant disease states including polycystic kidney disease and hypertension. Previous experiments investigating ciliary-mediated cellular mechanosensation have used either fluid flow chambers or micropipetting to elicit a biological response. The interpretation of these experiments in terms of the "ciliary hypothesis" has been difficult due the spatially distributed nature of the mechanical disturbance-several competing hypotheses regarding possible roles of primary cilium, glycocalyx, microvilli, cell junctions, and actin cytoskeleton exist. I report initial data using optical tweezers to manipulate individual primary cilia in an attempt to elicit a mechanotransduction response-specifically, the release of intracellular calcium. The advantage of using laser tweezers over previous work is that the applied disturbance is highly localized. I find that stimulation of a primary cilium elicits a response, while stimulation of the apical surface membrane does not. These results lend support to the hypothesis that the primary cilium mediates transduction of mechanical strain into a biochemical response in renal epithelia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.