ArticleJALA (Charlottesville, Va.)2010
Automated high-throughput microchannel assays for cell biology: Operational optimization and characterization.
Article in JALA (Charlottesville, Va.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Advances in passively driven microfluidics and lab-on-chip devices: a comprehensive literature review and patent analysis.RSC advances · 2020Review
- A Platform for High-Throughput Assessments of Environmental Multistressors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Adaptation of a Simple Microfluidic Platform for High-Dimensional Quantitative Morphological Analysis of Human Mesenchymal Stromal Cells on Polystyrene-Based Substrates.SLAS technology · 2017Article
- Multiwell capillarity-based microfluidic device for the study of 3D tumour tissue-2D endothelium interactions and drug screening in co-culture models.Scientific reports · 2017Article
- A novel 96well-formatted micro-gap plate enabling drug response profiling on primary tumour samples.Scientific reports · 2015Article
- High-density self-contained microfluidic KOALA kits for use by everyone.Journal of laboratory automation · 2015Article
- Cellular microenvironment dictates androgen production by murine fetal Leydig cells in primary culture.Biology of reproduction · 2014Article
- Vascular smooth muscle cell culture in microfluidic devices.Biomicrofluidics · 2014Article
- Characterizing asthma from a drop of blood using neutrophil chemotaxis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Kit-On-A-Lid-Assays for accessible self-contained cell assays.Lab on a chip · 2013Article
- Tubeless microfluidic angiogenesis assay with three-dimensional endothelial-lined microvessels.Biomaterials · 2013Article
- Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models.PloS one · 2013Article
- Article
- Automation of three-dimensional cell culture in arrayed microfluidic devices.Journal of laboratory automation · 2011Article
- Microfluidic cell culture and its application in high-throughput drug screening: cardiotoxicity assay for hERG channels.Journal of biomolecular screening · 2011Article
- Expanding the available assays: adapting and validating In-Cell Westerns in microfluidic devices for cell-based assays.Assay and drug development technologies · 2010Article
- Fundamentals of microfluidic cell culture in controlled microenvironments.Chemical Society reviews · 2010Review
- Optical oxygen sensors for applications in microfluidic cell culture.Sensors (Basel, Switzerland) · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Screening biological readouts in cell culture are increasing in frequency and throughput. In such assays, cell types may be rare and reagents or compounds may be expensive often resulting in a reduced number of conditions and/or replicates. "Tubeless" microfluidics offers a method to reduce this burden, as has been previously shown.1 In addition the In Cell Western (ICW) has recently been adapted to microfluidic cultures allowing high throughput analysis of immunocytochemistry (ICC) in microfluidic channels.2 Combining automated liquid handling in tubeless microfluidics with the ICW provides rapid and quantitative high throughput cell-based screens. Here we validate this platform using three parameters: operational robustness (pipetting reliability), cell seeding consistency, and cell staining consistency (both nuclear and antibody). Integration of liquid handling with microfluidics was found to be over 97% operationally robust. Cell seeding consistency between each microchannel and within each microchannel was found to be within a standard deviation of less than 5% and 6% respectively. Finally, through optimization of liquid handling steps, uniformity between all the channels was found for both nuclear and antibody staining. These results lay the foundation to perform most standard ICW assays using automated tubeless microfluidics.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.