ArticleJournal of bacteriology2010
Crystal structure of activated CheY1 from Helicobacter pylori.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Enhancing 3-O-methylfunicone production by endophytic Talaromyces pinophilus J6 through culture optimization and its anti-Helicobacter pylori activity.Archives of microbiology · 2026Article
- Article
- The evolutionary path of chemosensory and flagellar macromolecular machines in Campylobacterota.PLoS genetics · 2022Article
- Role of Position K+4 in the Phosphorylation and Dephosphorylation Reaction Kinetics of the CheY Response Regulator.Biochemistry · 2021Article
- The Divergent Key Residues of TwoMicroorganisms · 2021Article
- Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias ineLife · 2021Article
- Structure of the archaeal chemotaxis protein CheY in a domain-swapped dimeric conformation.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Colonization, localization, and inflammation: the roles of H. pylori chemotaxis in vivo.Current opinion in microbiology · 2018Review
- Probing Mechanistic Similarities between Response Regulator Signaling Proteins and Haloacid Dehalogenase Phosphatases.Biochemistry · 2015Article
- Molecular dynamics investigation of Helicobacter pylori chemotactic protein CheY1 and two mutants.Journal of molecular modeling · 2014Article
- Structural and functional aspects of the Helicobacter pylori secretome.World journal of gastroenterology · 2014Review
- Conformational barrier of CheY3 and inability of CheY4 to bind FliM control the flagellar motor action in Vibrio cholerae.PloS one · 2013Article
- Migration of chemotactic bacteria in soft agar: role of gel concentration.Biophysical journal · 2011Article
- Motility and chemotaxis in Campylobacter and Helicobacter .Annual review of microbiology · 2011Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemotaxis is an important virulence factor for Helicobacter pylori colonization and infection. The chemotactic system of H. pylori is marked by the presence of multiple response regulators: CheY1, one CheY-like-containing CheA protein (CheAY2), and three CheV proteins. Recent studies have demonstrated that these molecules play unique roles in the chemotactic signal transduction mechanisms of H. pylori. Here we report the crystal structures of BeF(3(-)-activated CheY1 from H. pylori resolved to 2.4 A. Structural comparison of CheY1 with active-site residues of BeF3(-)-bound CheY from Escherichia coli and fluorescence quenching experiments revealed the importance of Thr84 in the phosphotransfer reaction. Complementation assays using various nonchemotactic E. coli mutants and pull-down experiments demonstrated that CheY1 displays differential association with the flagellar motor in E. coli. The structural rearrangement of helix 5 and the C-terminal loop in CheY1 provide a different interaction surface for FliM. On the other hand, interaction of the CheA-P2 domain with CheY1, but not with CheY2/CheV proteins, underlines the preferential recognition of CheY1 by CheA in the phosphotransfer reaction. Our results provide the first structural insight into the features of the H. pylori chemotactic system as a model for Epsilonproteobacteria.
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