ArticleThe Journal of biological chemistry2010
Ring-shaped Rad51 paralog protein complexes bind Holliday junctions and replication forks as visualized by electron microscopy.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Novel insights into RAD51 activity and regulation during homologous recombination and DNA replication.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2016Review
- Rad51C-ATXN7 fusion gene expression in colorectal tumors.Molecular cancer · 2016Article
- Mammalian RAD51 paralogs protect nascent DNA at stalled forks and mediate replication restart.Nucleic acids research · 2015Article
- Overexpression of Rad51C splice variants in colorectal tumors.Oncotarget · 2015Article
- Roles of Rad51 paralogs for promoting homologous recombination in Leishmania infantum.Nucleic acids research · 2015Article
- DNA-pairing and annealing processes in homologous recombination and homology-directed repair.Cold Spring Harbor perspectives in biology · 2015Review
- DNA repair pathways in trypanosomatids: from DNA repair to drug resistance.Microbiology and molecular biology reviews : MMBR · 2014Review
- RAD51B Activity and Cell Cycle Regulation in Response to DNA Damage in Breast Cancer Cell Lines.Breast cancer : basic and clinical research · 2014Article
- Roles of XRCC2, RAD51B and RAD51D in RAD51-independent SSA recombination.PLoS genetics · 2013Article
- The HsRAD51B-HsRAD51C stabilizes the HsRAD51 nucleoprotein filament.DNA repair · 2013Article
- Biophysical and ultrastructural characterization of adeno-associated virus capsid uncoating and genome release.Journal of virology · 2013Article
- Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway.Molecular and cellular biology · 2013Article
- Human Rap1 interacts directly with telomeric DNA and regulates TRF2 localization at the telomere.The Journal of biological chemistry · 2012Article
- Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation.Molecular microbiology · 2011Article
- Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis.Nature structural & molecular biology · 2010Article
- DNA-binding properties of T4 UvsY recombination mediator protein: polynucleotide wrapping promotes high-affinity binding to single-stranded DNA.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In mammals, there are five Rad51 paralogs that form two distinct complexes in vivo. One complex is composed of Rad51B-Rad51C-Rad51D-Xrcc2 (BCDX2) and the other Rad51C-Xrcc3 (CX3). We co-expressed and purified human BCDX2 and CX3 protein complexes from insect cells and investigated their binding preferences and structure using transmission electron microscopy (TEM). We visualized the binding of BCDX2 and CX3 to DNA templates containing replication forks and Holliday junctions, intermediates observed during DNA replication and recombination, respectively. We show that both complexes bind with exceptionally high specificity to the DNA junctions with little binding observed elsewhere on the DNAs. Further analysis of the structure of free or DNA-bound BCDX2 and CX3 complexes revealed a multimeric ring structure whose subunits are arranged into a flat disc around a central channel. This work provides the first EM visualization of BCDX2 and CX3 binding to Holliday junctions and forked DNAs and suggests the complexes form ring-shaped structures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.