Evidence map›Paper›PMID 20207080›Full record

ArticlePain2010

Eccentric muscle contraction and stretching evoke mechanical hyperalgesia and modulate CGRP and P2X(3) expression in a functionally relevant manner.

Dean Dessem, Ranjinidevi Ambalavanar, Melena Evancho, Aicha Moutanni, Chandrasekhar Yallampalli, Guang Bai

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pain, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Dean DessemDepartment of Neural and Pain Sciences, University of Maryland, 650 West Baltimore Street, Baltimore, MD 21201, USA Graduate Program in Neuroscience, University of Maryland, Baltimore, MD, USA Department of Obstetrics and Gynecology, University of Texas, Galveston, TX 77555, USA.
Ranjinidevi Ambalavanar
Melena Evancho
Aicha Moutanni
Chandrasekhar Yallampalli
Guang Bai
University of Maryland, Baltimore · USTexas A&M University at Galveston · US

Funding

TOOTH MERCHANORECEPTORS IN JAW MOVEMENTR01DE010132 · NIDCR · UNIVERSITY OF MARYLAND BALTIMORE · PI DESSEM, DEAN A · 1991 to 2006
$2.0M
Neuronal Plasticity Related to TMD and FibromyalgiaR01DE015386 · NIDCR · UNIVERSITY OF MARYLAND BALTIMORE · PI DESSEM, DEAN A · 2003 to 2006
$1.2M
NIDCR NIH HHS DE10132NIDCR NIH HHS DE15386NIDCR NIH HHS R01 DE010132NIDCR NIH HHS R01 DE015386
6 · The paper itself

Abstract

Non-invasive, movement-based models were used to investigate muscle pain. In rats, the masseter muscle was rapidly stretched or electrically stimulated during forced lengthening to produce eccentric muscle contractions (EC). Both EC and stretching disrupted scattered myofibers and produced intramuscular plasma extravasation. Pro-inflammatory cytokines (IL-1beta, TNF-alpha, IL-6) and vascular endothelial growth factor (VEGF) were elevated in the masseter 24h following EC. At 48h, neutrophils increased and ED1 macrophages infiltrated myofibers while ED2 macrophages were abundant at 4d. Mechanical hyperalgesia was evident in the ipsilateral head 4h-4d after a single bout of EC and for 7d following multiple bouts (1 bout/d for 4d). Calcitonin gene-related peptide (CGRP) mRNA increased in the trigeminal ganglion 24h following EC while immunoreactive CGRP decreased. By 2d, CGRP-muscle afferent numbers equaled naive numbers implying that CGRP is released following EC and replenished within 2d. EC elevated P2X(3) mRNA and increased P2X(3) muscle afferent neuron number for 12d while electrical stimulation without muscle contraction altered neither CGRP nor P2X(3) mRNA levels. Muscle stretching produced hyperalgesia for 2d whereas contraction alone produced no hyperalgesia. Stretching increased CGRP mRNA at 24h but not CGRP-muscle afferent number at 2-12d. In contrast, stretching significantly increased the number of P2X(3) muscle afferent neurons for 12d. The sustained, elevated P2X(3) expression evoked by EC and stretching may enhance nociceptor responsiveness to ATP released during subsequent myofiber damage. Movement-based actions such as EC and muscle stretching produce unique tissue responses and modulate neuropeptide and nociceptive receptor expression in a manner particularly relevant to repeated muscle damage.

Indexed as

Adenosine TriphosphateAnimalsBiomarkersCalcitonin Gene-Related PeptideCytokinesDisease Models, AnimalHyperalgesiaInflammationMacrophagesMaleMuscle ContractionMuscle, SkeletalMuscle SpindlesMuscular DiseasesNeurons, AfferentRatsAdenosine TriphosphateBiomarkersCalcitonin Gene-Related PeptideCytokinesReceptors, Purinergic P2Receptors, Purinergic P2X3RNA, MessengerVascular Endothelial Growth Factor A

Identifiers

PMID20207080
PMCPMC2860684
OpenAlexW2047909602

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.