ArticleWorld journal of gastroenterology2010
Mechanisms mediating CCK-8S-induced contraction of proximal colon in guinea pigs.
Article in World journal of gastroenterology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 7 citations in OpenAlex.
- Ameliorative effects of atractylodin on intestinal inflammation and co-occurring dysmotility in both constipation and diarrhea prominent rats.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2017Article
- The Resveratrol-induced Relaxation of Cholecystokinin Octapeptide- or KCl-induced Tension in Male Guinea Pig Gallbladder Strips Is Mediated Through L-type Ca2+Channels.Journal of neurogastroenterology and motility · 2015Article
- Effects of ginsenoside Re on rat jejunal contractility.Journal of natural medicines · 2014Article
- Characteristics of deslanoside-induced modulation on jejunal contractility.World journal of gastroenterology · 2012Article
- Analgesic effect of Coptis chinensis rhizomes (Coptidis Rhizoma) extract on rat model of irritable bowel syndrome.Journal of ethnopharmacology · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the effects of sulfated cholecystokinin octapeptide (CCK-8S) on the contractile activity of guinea-pig proximal colon.
methodsProximal colonic smooth muscle (PCSM) strips were obtained from adult female guinea pigs and contractile response of PCSM strips was recorded using a polyphysiograph. PCSM cells were isolated by enzymatic digestion. Resting potential (RP), action potential (AP), large conductance potassium channel currents (I(BKCa)) and L-type calcium currents (I(Ca-L)) were recorded by patch-clamp techniques.
results(1) CCK-8S (10(-7) mol/L) enhanced the mean contractile amplitude of colonic circular muscle and longitudinal muscle (LM) strips by 56.53% + or - 11.92% (P = 0.038) and 65.93% + or - 12.98% (P = 0.019), respectively, as well as the mean frequency of LM by 31.69% + or - 13.58% (P = 0.023), which were significantly attenuated by pretreating strips with devazepide, nifedipine, iberiotoxin, thapsigargin (TG) and BAPTA-AM (BA) respectively; (2) CCK-8S (10(-7) mol/L) increased the AP amplitude by 38.6% + or - 3.2% (P = 0.015), decreased AP duration by 36.9% + or - 8.7% (P = 0.026), and depolarized the RP from -61.3 + or - 2.7 mV to -29.8 + or - 5.9 mV (P = 0.032); and (3) Compared with the normal control group, CCK-8S (10(-7) mol/L) enhanced the peak current of I(BKCa) by 18.7% + or - 2.1% (from 916 + or - 183 pA to 1088 + or - 226 pA; at +60 mV; P = 0.029), which was inhibited by respective pretreatment with iberiotoxin and devazepide. Additionally, CCK-8S (10(-7) mol/L) intensified the peak current of I(Ca-L) by 40% (from 60 + or - 8 pA to 84 + or - 11 pA; at +10 mV; P = 0.012), compared to the normal control group, which was apparently suppressed by respective pretreatment with nifedipine, devazepide, TG and BA. In the respective presence of heparin and staurosporine, CCK-8S did not significantly enhance I(BKCa) and I(Ca-L).
conclusionThe results suggest that CCK-8S promotes guinea-pig proximal colon contraction by CCK1 receptors, following activation of the inositol triphosphate-protein kinase C signal transduction pathway.
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