ArticleProteomics. Clinical applications2007
Investigation of an albumin-enriched fraction of human serum and its albuminome.
Article in Proteomics. Clinical applications, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed, 191 citations in OpenAlex.
- Vitronectin Modulates Plasma Aβ Oligomerization Propensity Within Altered Albumin Interactome Networks in Alzheimer's Disease.International journal of molecular sciences · 2026Article
- Relationship between serum glucose-to-albumin ratio and kidney stones among nondiabetic U.S. adults: a population-based study.Translational andrology and urology · 2025Article
- Article
- Non-human peptides revealed in blood reflect the composition of intestinal microbiota.BMC biology · 2024Article
- Composite Remineralization of Bone-Collagen Matrices by Low-Temperature Ceramics and Serum Albumin: A New Approach to the Creation of Highly Effective Osteoplastic Materials.Journal of functional biomaterials · 2024Article
- A Label-Free Optical Biosensor Based on an Array of Microring Resonators for the Detection of Human Serum Albumin.Sensors (Basel, Switzerland) · 2024Article
- Protein "purity," proteoforms, and the albuminome: critical observations on proteome and systems complexity.Frontiers in cell and developmental biology · 2024Article
- Unraveling the versatility of human serum albumin - A comprehensive review of its biological significance and therapeutic potential.Current research in structural biology · 2023Review
- An experimental workflow for enrichment of low abundant proteins from human serum for the discovery of serum biomarkers.Journal of biological methods · 2023Article
- The Interplay between Uremic Toxins and Albumin, Membrane Transporters and Drug Interaction.Toxins · 2022Review
- A Dual Workflow to Improve the Proteomic Coverage in Plasma Using Data-Independent Acquisition-MS.Journal of proteome research · 2020Article
- Deepening our understanding of HDL proteome.Expert review of proteomics · 2019Review
- A next generation setup for pre-fractionation of non-denatured proteins reveals diverse albumin proteoforms each carrying several post-translational modifications.Scientific reports · 2019Article
- Non-covalent albumin-binding ligands for extending the circulating half-life of small biotherapeutics.MedChemComm · 2019Review
- Clinical Proteomics for Post-Hematopoeitic Stem Cell Transplantation Outcomes.Proteomics. Clinical applications · 2019Review
- The anti-inflammatory effect of LMWF5A and N-acetyl kynurenine on macrophages: Involvement of aryl hydrocarbon receptor in mechanism of action.Biochemistry and biophysics reports · 2018Article
- Multiplexed Temporal Quantification of the Exercise-regulated Plasma Peptidome.Molecular & cellular proteomics : MCP · 2017Article
- Proteomic and network analysis of human serum albuminome by integrated use of quick crosslinking and two-step precipitation.Scientific reports · 2017Article
- Plasminogen promotes cholesterol efflux by the ABCA1 pathway.JCI insight · 2017Article
- Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The removal of albumin and other high abundance proteins is a routine first step in the analysis of serum and plasma proteomes. However, as albumin can bind proteins and peptides, there is a universal concern as to how the serum proteome is changed by the removal of albumin. To address this concern, the current study was designed to identify proteins and peptides removed from the serum during albumin depletion; to determine which of these are bound to albumin (rather than copurified) and whether the bound proteins are intact proteins or peptide fragments. Sequential, independent analyses including both anti-albumin antibody (anti-HSA) affinity chromatography and SEC were used to isolate albumin-bound proteins. RP-HPLC and 1-D SDS-PAGE were then used to further separate the proteins prior to identification by MS/MS. Finally, whole protein molecular weight (MW) MS measurements coupled with protein coverage obtained by MS were combined to assess whether the bound proteins were intact or peptide fragments. Combining the results from multiple approaches, 35 proteins, of which 24 are intact, were found to be associated with albumin, and they include both known high and low abundance proteins.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.