ArticleNature protocols2010
NMR-based metabolomic analysis of plants.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 258 papers.
What it found
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Who cites it
258 citing papers in PubMed.
- Metabolomic Mapping of Greek Olive Leaves by Untargeted NMR-Based Profiling and LC-HRMS Dereplication.Analytical science advances · 2026Article
- Synergistic lipase inhibition by Garcinia parvifolia metabolites: aMetabolomics : Official journal of the Metabolomic Society · 2026Article
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- Allelochemical Potential ofPlants (Basel, Switzerland) · 2026Article
- Comparing the Effect of Biochar and Poultry Manure on the Metabolomic Profile of Tomatoes (Metabolites · 2026Article
- Antigonococcal Activity and Cytotoxicity Screening of Three Ziziphus Species Indigenous to South Africa.Chemistry & biodiversity · 2026Article
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- Rosemary extract primes cultivar-dependent defense responses in potato against pathogen attack.Plant cell reports · 2026Article
- Metabolomic Profiling of Seasonal Katsumadin Production inMolecules (Basel, Switzerland) · 2026Article
- Metabolomics, Molecular Networking and Phytochemical Investigation ofMolecules (Basel, Switzerland) · 2026Article
- Optimization of carbon and nitrogen utilization for enhanced photosynthesis, biomass and yield by coexpression of phosphoenolpyruvate carboxylase, aspartate aminotransferase and glutamine synthetase in Brassica juncea.Plant biotechnology journal · 2026Article
- Combined Transcriptomic and Metabolomic Analysis Reveals an Ethylene-Activated Regulatory Model on Monoterpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus.Plant biotechnology journal · 2026Article
- Harnessing phytochemicals for engineering health solutions.Human genomics · 2026Review
- Enhancing detection of low‑abundance metabolites in proton NMR through band‑selective suppression and presaturation.Natural products and bioprospecting · 2026Article
- Nitrate availability influences powdery mildew resistance and secondary metabolism inFrontiers in plant science · 2026Article
- AnnoMe: user-defined classification of HR-MS/MS spectra for natural product discovery.Bioinformatics advances · 2026Article
- Gas Chromatography-Mass Spectrometry (GC-MS) in the Plant Metabolomics Toolbox: Sample Preparation and Instrumental Analysis.Biomolecules · 2025Review
- Complementarity assessment of NMR and LC-HRMS profiling in natural products: an application on mastic leaves.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- NMR and GC-MS based metabolic profiling, total phenolic content, antibacterial, antioxidant, anticancer and In-silico antiviral activity of Origanum ramonense plant.Scientific reports · 2025Article
- Design and Analysis of Untargeted Metabolomics Experiments.Current protocols · 2025Article
198 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear magnetic resonance (NMR)-based metabolomics has many applications in plant science. Metabolomics can be used in functional genomics and to differentiate plants from different origin, or after different treatments. In this protocol, the following steps of plant metabolomics using NMR spectroscopy are described: sample preparation (freeze drying followed by extraction by ultrasonication with 1:1 CD(3)OD:KH(2)PO(4) buffer in D(2)O), NMR analysis (standard (1)H, J-resolved, (1)H-(1)H correlation spectroscopy (COSY) and heteronuclear multiple bond correlation (HMBC)) and chemometric methods. The main advantage of NMR metabolomic analysis is the possibility of identifying metabolites by comparing NMR data with references or by structure elucidation using two-dimensional NMR. This protocol is particularly suited for the analysis of secondary metabolites such as phenolic compounds (usually abundant in plants), and for primary metabolites (e.g., sugars and amino acids). This procedure is rapid; it takes not more than 30 min for sample preparation (multiple parallel) and a further 10 min for NMR spectrum acquisition.
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