ArticleNature protocols2010
Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 335 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
335 citing papers in PubMed, 694 citations in OpenAlex.
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- Fabrication of High-Density Multimodal Neural Probes Based on Heterogeneously Integrated CMOS.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cell type-specific barcoding reveals the single-neuron projectional architecture of the mouse midbrain dopaminergic system.bioRxiv : the preprint server for biology · 2026Article
- Early life adversity impairs visually evoked innate defensive behaviors via oxytocin signaling.Communications biology · 2026Article
- Cortical Response to Acute Implantation of the Utah Optrode Array in Macaque Cortex.Advanced healthcare materials · 2026Article
- Regulation of Calcium Ion Channels with Conjugated Molecules for Modulating Biological Functions.ACS central science · 2025Review
- Neural network tissue and myocytes co-culture system reveals effects of different myocytes on postnatal development of spinal cord neural network.Cellular and molecular life sciences : CMLS · 2025Article
- Glial Plasticity and Metabolic Stability After Knockdown of Astrocytic Cx43 in the Dorsal Vagal Complex.Cells · 2025Article
- A Photoresponsive Hybrid of Viruses and Supramolecular Peptide Fibers for Multidimensional Control of Patterning and Infection.Angewandte Chemie (International ed. in English) · 2025Article
- Integrating optical neuroscience tools into touchscreen operant systems.Nature protocols · 2025Review
- Using Manganese-Enhanced MRI to visualize Magnetogenetic-based Neuromodulation.bioRxiv : the preprint server for biology · 2025Article
- In vivo functional screens revealScience advances · 2025Article
- Reply to: A curious concept of CNS clearance.Nature neuroscience · 2025Article
- Devaluing memories of reward: a case for dopamine.Communications biology · 2025Article
- Multimodal evaluation of network activity and optogenetic interventions in human hippocampal slices.Nature neuroscience · 2024Article
- An Integrated Optogenetic and Bioelectronic Platform for Regulating Cardiomyocyte Function.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
275 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Elucidation of the neural substrates underlying complex animal behaviors depends on precise activity control tools, as well as compatible readout methods. Recent developments in optogenetics have addressed this need, opening up new possibilities for systems neuroscience. Interrogation of even deep neural circuits can be conducted by directly probing the necessity and sufficiency of defined circuit elements with millisecond-scale, cell type-specific optical perturbations, coupled with suitable readouts such as electrophysiology, optical circuit dynamics measures and freely moving behavior in mammals. Here we collect in detail our strategies for delivering microbial opsin genes to deep mammalian brain structures in vivo, along with protocols for integrating the resulting optical control with compatible readouts (electrophysiological, optical and behavioral). The procedures described here, from initial virus preparation to systems-level functional readout, can be completed within 4-5 weeks. Together, these methods may help in providing circuit-level insight into the dynamics underlying complex mammalian behaviors in health and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.