Evidence map›Paper›PMID 20203291›Full record

ArticleAmerican journal of respiratory cell and molecular biology2010

MARCKS and related chaperones bind to unconventional myosin V isoforms in airway epithelial cells.

Ko-Wei Lin, Shijing Fang, Joungjoa Park, Anne L Crews, Kenneth B Adler

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of respiratory cell and molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. MARCKS and Lung Disease.American journal of respiratory cell and molecular biology · 2019
    Review
  3. Review
  4. Multiple roles for the actin cytoskeleton during regulated exocytosis.Cellular and molecular life sciences : CMLS · 2013
    Review
  5. Article
  6. MARCKS and HSP70 interactions regulate mucin secretion by human airway epithelial cells in vitro.American journal of physiology. Lung cellular and molecular physiology · 2013
    Article
  7. Calpain and MARCKS protein regulation of airway mucin secretion.Pulmonary pharmacology & therapeutics · 2012
    Article
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ko-Wei LinDepartment of Molecular Biomedical Sciences, North Carolina State University, Raleigh, 27606, USA.
Shijing Fang
Joungjoa Park
Anne L Crews
Kenneth B Adler
North Carolina State University · US

Funding

Mechanism of Oxidant-Induced Respiratory Mucin SecretionR37HL036982 · NHLBI · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI ADLER, KENNETH BRUCE · 2004 to 2013
$4.0M
NHLBI NIH HHS R37 HL36982
6 · The paper itself

Abstract

We have shown previously that myristoylated alanine-rich C kinase substrate (MARCKS) is a key regulatory molecule in the process of mucin secretion by airway epithelial cells, and that part of the secretory mechanism involves intracellular associations of MARCKS with specific chaperones: heat shock protein 70 (Hsp70) and cysteine string protein (CSP). Here, we report that MARCKS also interacts with unconventional myosin isoforms within these cells, and further molecular interactions between MARCKS and these chaperones/cytoskeletal proteins are elucidated. Primary human bronchial epithelial cells and the HBE1 cell line both expressed myosin V and VI proteins, and both MARCKS and CSP were shown to bind to myosin V, specifically Va and Vc. This binding was enhanced by exposing the cells to phorbol-12-myristate-13-acetate, an activator of protein kinase C and stimulator of mucin secretion. Binding of MARCKS, Hsp70, and CSP was further investigated by His-tagged pull down assays of purified recombinant proteins and multiple transfections of HBE1 cells with fusion proteins (MARCKS-HA; Flag-Hsp70; c-Myc-CSP) and immunoprecipitation. The results showed that MARCKS binds directly to Hsp70, and that Hsp70 binds directly to CSP, but that MARCKS binding to CSP appears to require the presence of Hsp70. Interrelated binding(s) of MARCKS, chaperones, and unconventional myosin isoforms may be integral to the mucin secretion process.

Indexed as

Gene Expression RegulationBronchiEpithelial CellsHSP70 Heat-Shock ProteinsHumansIntracellular Signaling Peptides and ProteinsMembrane ProteinsModels, BiologicalMolecular ChaperonesMucinsMyosin Heavy ChainsMyosin Type VMyristoylated Alanine-Rich C Kinase SubstrateProtein BindingProtein IsoformsTetradecanoylphorbol AcetateHSP70 Heat-Shock ProteinsIntracellular Signaling Peptides and ProteinsMARCKS protein, humanMembrane ProteinsMolecular ChaperonesMucinsMyosin Heavy ChainsMyosin Type Vmyosin VIMyristoylated Alanine-Rich C Kinase SubstrateProtein IsoformsTetradecanoylphorbol Acetate

Identifiers

PMID20203291
PMCPMC2937227
OpenAlexW2145722990

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.