ArticleJournal of applied physiology (Bethesda, Md. : 1985)2010
A new method to study in vivo protein synthesis in slow- and fast-twitch muscle fibers and initial measurements in humans.
Article in Journal of applied physiology (Bethesda, Md. : 1985), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 45 citations in OpenAlex.
- Post-exercise protein synthesis rates are only marginally higher in type I compared with type II muscle fibres following resistance-type exercise.European journal of applied physiology · 2011Trial
- Brief ambulatory reloading elevates muscle protein synthesis but does not prevent disuse atrophy in hindlimb-unloaded rats.Biochemistry and biophysics reports · 2025Article
- Human skeletal muscle-specific hypertrophy with exercise training and aging: a comprehensive review.Journal of applied physiology (Bethesda, Md. : 1985) · 2025Review
- Integrated single-cell multiome analysis reveals muscle fiber-type gene regulatory circuitry modulated by endurance exercise.Genome research · 2025Article
- Endurance exercise with reduced muscle glycogen content influences substrate utilization and attenuates acute mTORC1- and autophagic signaling in human type I and type II muscle fibers.Skeletal muscle · 2025Article
- The utility-and limitations-of the rodent synergist ablation model in examining mechanisms of skeletal muscle hypertrophy.American journal of physiology. Cell physiology · 2024Review
- Fast and slow muscle fiber transcriptome dynamics with lifelong endurance exercise.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Muscle fiber phenotype: a culprit of abnormal metabolism and function in skeletal muscle of humans with obesity.American journal of physiology. Endocrinology and metabolism · 2023Review
- Mechanisms of mechanical overload-induced skeletal muscle hypertrophy: current understanding and future directions.Physiological reviews · 2023Review
- Postabsorptive muscle protein synthesis is higher in outpatients as compared to inpatients.American journal of physiology. Endocrinology and metabolism · 2023Article
- Muscle memory: myonuclear accretion, maintenance, morphology, and miRNA levels with training and detraining in adult mice.Journal of cachexia, sarcopenia and muscle · 2020Article
- Fiber typing human skeletal muscle with fluorescent immunohistochemistry.Journal of applied physiology (Bethesda, Md. : 1985) · 2019Review
- mTORC1 Signaling in Individual Human Muscle Fibers Following Resistance Exercise in Combination With Intake of Essential Amino Acids.Frontiers in nutrition · 2019Article
- Muscle health and performance in monozygotic twins with 30 years of discordant exercise habits.European journal of applied physiology · 2018Article
- Starring or Supporting Role? Satellite Cells and Skeletal Muscle Fiber Size Regulation.Physiology (Bethesda, Md.) · 2018Review
- Normal Ribosomal Biogenesis but Shortened Protein Synthetic Response to Acute Eccentric Resistance Exercise in Old Skeletal Muscle.Frontiers in physiology · 2018Article
- Mouse soleus (slow) muscle shows greater intramyocellular lipid droplet accumulation than EDL (fast) muscle: fiber type-specific analysis.Journal of muscle research and cell motility · 2017Article
- DNA methylation assessment from human slow- and fast-twitch skeletal muscle fibers.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Article
- Dihydrotestosterone treatment rescues the decline in protein synthesis as a result of sarcopenia in isolated mouse skeletal muscle fibres.Journal of cachexia, sarcopenia and muscle · 2017Article
- Linking Cancer Cachexia-Induced Anabolic Resistance to Skeletal Muscle Oxidative Metabolism.Oxidative medicine and cellular longevity · 2017Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The aim of this study was to develop an approach to directly assess protein fractional synthesis rate (FSR) in isolated human muscle fibers in a fiber type-specific fashion. Individual muscle fibers were isolated from biopsies of the vastus lateralis (VL) and soleus (SOL) obtained from eight young men during a primed, continuous infusion of [5,5,5-(2)H3]leucine performed under basal conditions. To determine mixed protein FSR, a portion of each fiber was used to identify fiber type, fibers of the same type were pooled, and the [5,5,5-(2)H3]leucine enrichment was determined via GC-MS. Processing isolated slow-twitch [myosin heavy chain (MHC) I] and fast-twitch (MHC IIa) fibers for mixed protein bound [5,5,5-(2)H3]leucine enrichment yielded mass ion chromatographic peaks that were similar in shape, abundance, and measurement reliability as tissue homogenates. In the VL, MHC I fibers exhibited a 33% faster (P<0.05) mixed protein FSR compared with MHC IIa fibers (0.068+/-0.006 vs. 0.051+/-0.003%/h). MHC I fibers from the SOL (0.060+/-0.005%/h) and MHC I fibers from the VL displayed similar (P>0.05) mixed protein FSR. Feasibility of processing isolated human muscle fibers for analysis of myofibrillar protein [5,5,5-(2)H3]leucine enrichment was also confirmed in non-fiber-typed pooled fibers from the VL. These methods can be applied to the study of fiber type-specific responses in human skeletal muscle. The need for this level of investigation is underscored by the different contributions of each fiber type to whole muscle function and the numerous distinct adaptive functional and metabolic changes in MHC I and MHC II fibers originating from the same muscle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.