ArticleProceedings of the National Academy of Sciences of the United States of America2010
In situ assembly of macromolecular complexes triggered by light.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A Cholesterol Analogue for Cell-Surface Enzyme Display.Angewandte Chemie (International ed. in English) · 2026Article
- Three-Color Protein Photolithography with Green, Red, and Far-Red Light.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Peptide-Based Low Molecular Weight Photosensitive Supramolecular Gelators.Gels (Basel, Switzerland) · 2022Review
- Engineering Proteins at Interfaces: From Complementary Characterization to Material Surfaces with Designed Functions.Angewandte Chemie (International ed. in English) · 2018Review
- Controlling Protein Surface Orientation by Strategic Placement of Oligo-Histidine Tags.ACS nano · 2017Article
- Light-Controlled Cellular Internalization and Cytotoxicity of Nucleic Acid-Binding Agents: Studies in Vitro and in Zebrafish Embryos.Chembiochem : a European journal of chemical biology · 2016Article
- Photocleavable linker for the patterning of bioactive molecules.Scientific reports · 2015Article
- Wavelength-controlled photocleavage for the orthogonal and sequential release of multiple proteins.Angewandte Chemie (International ed. in English) · 2013Article
- Cobalt(III) as a stable and inert mediator ion between NTA and His6-tagged proteins.Angewandte Chemie (International ed. in English) · 2013Article
- Spatiotemporal control of microtubule nucleation and assembly using magnetic nanoparticles.Nature nanotechnology · 2013Article
- Direct biomolecule binding on nonfouling surfaces via newly discovered supramolecular self-assembly of lysozyme under physiological conditions.Macromolecular bioscience · 2012Article
- Nanobiochips.Cellular and molecular life sciences : CMLS · 2012Review
- His-tags lighten up and lose their inhibitions.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemical biology aims for a perfect control of protein complexes in time and space by their site-specific labeling, manipulation, and structured organization. Here we developed a self-inactivated, lock-and-key recognition element whose binding to His-tagged proteins can be triggered by light from zero to nanomolar affinity. Activation is achieved by photocleavage of a tethered intramolecular ligand arming a multivalent chelator head for high-affinity protein interaction. We demonstrate site-specific, stable, and reversible binding in solution as well as at interfaces controlled by light with high temporal and spatial resolution. Multiplexed organization of protein complexes is realized by an iterative in situ writing and binding process via laser scanning microscopy. This light-triggered molecular recognition should allow for a spatiotemporal control of protein-protein interactions and cellular processes by light-triggered protein clustering.
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