ArticleMolecular biology of the cell2010
Requirements for transitional endoplasmic reticulum site structure and function in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 61 citations in OpenAlex.
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- Scp160 deletion suppresses TDP-43 aggregation and toxicity inMicrobial cell (Graz, Austria) · 2026Article
- Establishment of the phagophore-ERES membrane contact site initiates phagophore elongation.Nature structural & molecular biology · 2025Article
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- Sorting and Export of Proteins at the Endoplasmic Reticulum.Cold Spring Harbor perspectives in biology · 2023Review
- Sec16 and Sed4 interdependently function as interaction and localization partners at ER exit sites.Journal of cell science · 2023Article
- Vesicular and uncoated Rab1-dependent cargo carriers facilitate ER to Golgi transport.Journal of cell science · 2020Article
- Cargo crowding contributes to sorting stringency in COPII vesicles.The Journal of cell biology · 2020Article
- Article
- The Role of Secretory Pathways inJournal of fungi (Basel, Switzerland) · 2020Review
- Sec16 function in ER export and autophagy is independent of its phosphorylation inMolecular biology of the cell · 2020Article
- Export Control: Post-transcriptional Regulation of the COPII Trafficking Pathway.Frontiers in cell and developmental biology · 2020Review
- Lipid droplet and peroxisome biogenesis occur at the same ER subdomains.Nature communications · 2018Article
- Lysophospholipids Facilitate COPII Vesicle Formation.Current biology : CB · 2018Article
- Coordinate Regulation of Yeast Sterol Regulatory Element-binding Protein (SREBP) and Mga2 Transcription Factors.The Journal of biological chemistry · 2017Article
- Regulation of ER-Golgi Transport Dynamics by GTPases in Budding Yeast.Frontiers in cell and developmental biology · 2017Review
- A family of membrane-shaping proteins at ER subdomains regulates pre-peroxisomal vesicle biogenesis.The Journal of cell biology · 2016Article
- Article
- Three-dimensional and immune electron microscopic analysis of the secretory pathway in Saccharomyces cerevisiae.Histochemistry and cell biology · 2016Article
- ER arrival sites for COPI vesicles localize to hotspots of membrane trafficking.The EMBO journal · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Secretory proteins are exported from the endoplasmic reticulum (ER) at specialized regions known as the transitional ER (tER). Coat protein complex II (COPII) proteins are enriched at tER sites, although the mechanisms underlying tER site assembly and maintenance are not understood. Here, we investigated the dynamic properties of tER sites in Saccharomyces cerevisiae and probed protein and lipid requirements for tER site structure and function. Thermosensitive sec12 and sec16 mutations caused a collapse of tER sites in a manner that depended on nascent secretory cargo. Continual fatty acid synthesis was required for ER export and for normal tER site structure, whereas inhibition of sterol and ceramide synthesis produced minor effects. An in vitro assay to monitor assembly of Sec23p-green fluorescent protein at tER sites was established to directly test requirements. tER sites remained active for approximately 10 min in vitro and depended on Sec12p function. Bulk phospholipids were also required for tER site structure and function in vitro, whereas depletion of phophatidylinositol selectively inhibited coat protein complex II (COPII) budding but not assembly of tER site structures. These results indicate that tER sites persist through relatively stringent treatments in which COPII budding was strongly inhibited. We propose that tER site structures are stable elements that are assembled on an underlying protein and lipid scaffold.
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