ArticlePlant physiology2010
The development of an efficient multipurpose bean pod mottle virus viral vector set for foreign gene expression and RNA silencing.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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91 citing papers in PubMed, 222 citations in OpenAlex.
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- The Resistance of Soybean Variety Heinong 84 to Apple Latent Spherical Virus Is Controlled by Two Genetic Loci.International journal of molecular sciences · 2024Article
- High production of recombinant protein using geminivirus-based deconstructed vectors inFrontiers in plant science · 2024Article
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- Soybean 40S Ribosomal Protein S8 (GmRPS8) Interacts with 6K1 Protein and Contributes to Soybean Susceptibility toViruses · 2023Article
- SilencingInternational journal of molecular sciences · 2023Article
- Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris.Virology journal · 2023Article
- Viral synergism suppresses R gene-mediated resistance by impairing downstream defense mechanisms in soybean.Plant physiology · 2023Article
- A molecular toolkit to boost functional genomic studies in transformation-recalcitrant vegetable legumes.Horticulture research · 2023Article
- SoybeanInternational journal of molecular sciences · 2023Article
- A cowpea severe mosaic virus-based vector simplifies virus-induced gene silencing and foreign protein expression in soybean.Plant methods · 2022Article
31 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant viral vectors are valuable tools for heterologous gene expression, and because of virus-induced gene silencing (VIGS), they also have important applications as reverse genetics tools for gene function studies. Viral vectors are especially useful for plants such as soybean (Glycine max) that are recalcitrant to transformation. Previously, two generations of bean pod mottle virus (BPMV; genus Comovirus) vectors have been developed for overexpressing and silencing genes in soybean. However, the design of the previous vectors imposes constraints that limit their utility. For example, VIGS target sequences must be expressed as fusion proteins in the same reading frame as the viral polyprotein. This requirement limits the design of VIGS target sequences to open reading frames. Furthermore, expression of multiple genes or simultaneous silencing of one gene and expression of another was not possible. To overcome these and other issues, a new BPMV-based vector system was developed to facilitate a variety of applications for gene function studies in soybean as well as in common bean (Phaseolus vulgaris). These vectors are designed for simultaneous expression of multiple foreign genes, insertion of noncoding/antisense sequences, and simultaneous expression and silencing. The simultaneous expression of green fluorescent protein and silencing of phytoene desaturase shows that marker gene-assisted silencing is feasible. These results demonstrate the utility of this BPMV vector set for a wide range of applications in soybean and common bean, and they have implications for improvement of other plant virus-based vector systems.
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