ArticlePlant physiology2010
MISSA is a highly efficient in vivo DNA assembly method for plant multiple-gene transformation.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Bxb1 and ParA recombinase-based gene assembly for multiple genes expression in plant cells.Plant physiology · 2026Article
- Ectopic overexpression ofFrontiers in plant science · 2024Article
- Article
- Plant Synthetic Metabolic Engineering for Enhancing Crop Nutritional Quality.Plant communications · 2020Review
- MISSA 2.0: an updated synthetic biology toolbox for assembly of orthogonal CRISPR/Cas systems.Scientific reports · 2017Article
- Co-overexpression of the Constitutively Active Form of OsbZIP46 and ABA-Activated Protein Kinase SAPK6 Improves Drought and Temperature Stress Resistance in Rice.Frontiers in plant science · 2017Article
- Plant artificial chromosome technology and its potential application in genetic engineering.Plant biotechnology journal · 2016Review
- Isolation and functional characterization of a novel rice constitutive promoter.Plant cell reports · 2014Article
- Engineering crassulacean acid metabolism to improve water-use efficiency.Trends in plant science · 2014Review
- Co-expression of the Arabidopsis SOS genes enhances salt tolerance in transgenic tall fescue (Festuca arundinacea Schreb.).Protoplasma · 2014Article
- Advanced genetic tools for plant biotechnology.Nature reviews. Genetics · 2013Review
- Creation and validation of a widely applicable multiple gene transfer vector system for stable transformation in plant.Plant molecular biology · 2013Article
- GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology.Plant physiology · 2013Article
- Delivery of multiple transgenes to plant cells by an improved version of MultiRound Gateway technology.Transgenic research · 2013Article
- Parallel in vivo DNA assembly by recombination: experimental demonstration and theoretical approaches.PloS one · 2013Article
- Zinc finger nuclease and homing endonuclease-mediated assembly of multigene plant transformation vectors.Plant physiology · 2012Article
- GoldenBraid: an iterative cloning system for standardized assembly of reusable genetic modules.PloS one · 2011Article
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a highly efficient in vivo DNA assembly method, multiple-round in vivo site-specific assembly (MISSA), which facilitates plant multiple-gene transformation. MISSA is based on conjugational transfer, which is driven by donor strains, and two in vivo site-specific recombination events, which are mediated by inducible Cre recombinase and phage lambda site-specific recombination proteins in recipient strains, to enable in vivo transfer and in vivo assembly of multiple transgenic DNA. The assembly reactions can be performed circularly and iteratively through alternate use of the two specially designed donor vectors. As proof-of-principle experiments, we constructed a few plant multigene binary vectors. One of these vectors was generated by 15 rounds of MISSA reactions and was confirmed in transgenic Arabidopsis (Arabidopsis thaliana). As MISSA simplifies the tedious and time-consuming in vitro manipulations to a simple mixing of bacterial strains, it will greatly save time, effort, and expense associated with the assembly of multiple transgenic or synthetic DNA. The principle that underlies MISSA is applicable to engineering polygenic traits, biosynthetic pathways, or protein complexes in all organisms, such as Escherichia coli, yeast, plants, and animals. MISSA also has potential applications in synthetic biology, whether for basic theory or for applied biotechnology, aiming at the assembly of genetic pathways for the production of biofuels, pharmaceuticals, and industrial compounds from natural or synthetic DNA.
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