ArticleMolecular microbiology2010
Distinct roles of ppGpp and DksA in Legionella pneumophila differentiation.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 76 citations in OpenAlex.
- Virulence Regulation inMicroorganisms · 2025Review
- Transcription factors DksA and PsrA are synergistic contributors toMicrobiology (Reading, England) · 2025Article
- InSeq analysis of definedmBio · 2024Article
- Ecology ofBiofilm · 2024Review
- Metabolism and physiology of pathogenic bacterial obligate intracellular parasites.Frontiers in cellular and infection microbiology · 2024Review
- Article
- Development of heat-shock resistance inApplied and environmental microbiology · 2023Article
- Legionella pneumophila Cas2 Promotes the Expression of Small Heat Shock Protein C2 That Is Required for Thermal Tolerance and Optimal Intracellular Infection.Infection and immunity · 2022Article
- The Pseudomonas aeruginosa DksA1 protein is involved in HScientific reports · 2022Article
- Expression and structure of the Chlamydia trachomatis DksA ortholog.Pathogens and disease · 2022Article
- Pathophysiology of enteropathogenicJournal of veterinary science · 2022Review
- Many birds with one stone: targeting the (p)ppGpp signaling pathway of bacteria to improve antimicrobial therapy.Biophysical reviews · 2021Review
- Quorum sensing governs a transmissive Legionella subpopulation at the pathogen vacuole periphery.EMBO reports · 2021Article
- Quorum sensing controls persistence, resuscitation, and virulence of Legionella subpopulations in biofilms.The ISME journal · 2021Article
- Gene Regulation and Transcriptomics.Current issues in molecular biology · 2021Article
- Dual RNA-seq provides novel insight into the roles ofZoological research · 2020Article
- DksA Controls the Response of the Lyme Disease SpirocheteJournal of bacteriology · 2019Article
- Transcriptomic Changes ofFrontiers in cellular and infection microbiology · 2019Article
- Guanosine tetraphosphate relieves the negative regulation of Salmonella pathogenicity island-2 gene transcription exerted by the AT-rich ssrA discriminator region.Scientific reports · 2018Article
- A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
To transit between hosts, intracellular Legionella pneumophila transform into a motile, infectious, transmissive state. Here we exploit the pathogen's life cycle to examine how guanosine tetraphosphate (ppGpp) and DksA cooperate to govern bacterial differentiation. Transcriptional profiling revealed that during transmission alarmone accumulation increases the mRNA for flagellar and Type IV-secretion components, secreted host effectors and regulators, and decreases transcripts for translation, membrane modification and ATP synthesis machinery. DksA is critical for differentiation, since mutants are defective for stationary phase survival, flagellar gene activation, lysosome avoidance and macrophage cytotoxicity. The roles of ppGpp and DksA depend on the context. For macrophage transmission, ppGpp is essential, whereas DksA is dispensable, indicating that ppGpp can act autonomously. In broth, DksA promotes differentiation when ppGpp levels increase, or during fatty acid stress, as judged by flaA expression and evasion of degradation by macrophages. For flagella morphogenesis, DksA is required for basal fliA (sigma(28)) promoter activity. When alarmone levels increase, DksA cooperates with ppGpp to generate a pulse of Class II rod RNA or to amplify the Class III sigma factor and Class IV flagellin RNAs. Thus, DksA responds to the level of ppGpp and other stress signals to co-ordinate L. pneumophila differentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.