Evidence map›Paper›PMID 20198411›Full record

ArticleJournal of fluorescence2010

Styryl dyes as two-photon excited fluorescent probes for DNA detection and two-photon laser scanning fluorescence microscopy of living cells.

Valentyna P Tokar, Mykhaylo Yu Losytskyy, Tymish Y Ohulchanskyy, Dmytro V Kryvorotenko, Vladyslava B Kovalska, Anatoliy O Balanda, Igor M Dmytruk, Vadym M Prokopets, Sergiy M Yarmoluk, Valeriy M Yashchuk

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Article in Journal of fluorescence, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Valentyna P TokarInstitute of Molecular Biology and Genetics of NASU, 150 Zabolotnogo Str., 03143, Kyiv, Ukraine.
Mykhaylo Yu Losytskyy
Tymish Y Ohulchanskyy
Dmytro V Kryvorotenko
Vladyslava B Kovalska
Anatoliy O Balanda
Igor M Dmytruk
Vadym M Prokopets
Sergiy M Yarmoluk
Valeriy M Yashchuk
Institute of Molecular Biology and Genetics · UATaras Shevchenko National University of Kyiv · UAUniversity at Buffalo, State University of New York · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Spectral-fluorescent properties of benzothiazole styryl monomer (Bos-3) and homodimer (DBos-21) dyes in presence of DNA were studied. The dyes enhance their fluorescence intensity in 2-3 orders of magnitude upon interaction with DNA. Studied styrylcyanines in DNA presence demonstrate rather high values of two-photon absorption (TPA) cross-section, which are comparable with the values of TPA cross section of the rhodamine dyes. An applicability of the styrylcyanines as probes for the fluorescence microscopy of living cells was studied. It was shown that both dyes are cell-permeable but homodimer dye DBos-21 produces noticeably brighter staining of HeLa cells comparing with monomer dye Bos-3. Molecules of DBos-21 initially bind to the nucleic acids-containing cell organelles (presumable mitochondria) and are able to penetrate into the cell nucleus. Thus, homodimer styryl DBos-21 dye is viewed as efficient stain for single-photon and two-photon excitation fluorescence imaging of living cells.

Indexed as

LasersPhotonsAbsorptionAnimalsCell SurvivalDimerizationDNAFluorescent DyesHeLa CellsHumansMicroscopy, FluorescenceStyrenesThiazoles2-styrylbenzothiazoleDNAFluorescent DyesStyrenesThiazoles

Identifiers

PMID20198411
OpenAlexW2115251001

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.