ArticleJournal of molecular evolution2010
Enhancing the prebiotic relevance of a set of covalently self-assembling, autorecombining RNAs through in vitro selection.
Article in Journal of molecular evolution, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- From formamide to RNA, the path is tenuous but continuous.Life (Basel, Switzerland) · 2015Article
- The RNA World: molecular cooperation at the origins of life.Nature reviews. Genetics · 2015Review
- Trans-splicing with the group I intron ribozyme from Azoarcus.RNA (New York, N.Y.) · 2014Article
- Fixation and accumulation of thermotolerant catalytic competence of a pair of ligase ribozymes through complex formation and cross ligation.Journal of molecular evolution · 2013Article
- The evolution of enzyme specificity in the metabolic replicator model of prebiotic evolution.PloS one · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An in vitro form of the self-splicing group-I intron interrupting the Azoarcus tRNA(Ile) was shortened by ~10% with the removal of helix P6a. This deletion reduced the reverse-splicing activity of the ribozyme about 10-fold. Through in vitro selection, this activity was restored in several low-error mutants. A number of mutations were found that improved reverse-splicing activity through both increased k (obs) and better folding. The deletion mutant could be fragmented into as many as three discrete pieces, which, when incubated together, were capable of covalent self-assembly through energy-neutral transesterification reactions, a process called autorecombination. A subset of the mutations identified through in vitro selection for reverse-splicing were exaptations in that they were also shown to augment the autorecombination reactions, leading to higher yields of covalently self-assembled products, making this the smallest such system yet discovered.
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