ArticleJournal of cell science2010
Early initiation of a replication origin tethered at the nuclear periphery.
Article in Journal of cell science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Replication program of a single-chromosome budding yeast strain.Nucleic acids research · 2025Article
- Where and when to start: Regulating DNA replication origin activity in eukaryotic genomes.Nucleus (Austin, Tex.) · 2023Review
- Aberrant association of chromatin with nuclear periphery induced by Rif1 leads to mitotic defect.Life science alliance · 2023Article
- Rif1-Dependent Control of Replication Timing.Genes · 2022Review
- Mapping replication timing domains genome wide in single mammalian cells with single-cell DNA replication sequencing.Nature protocols · 2020Article
- Article
- Review
- Shelterin promotes tethering of late replication origins to telomeres for replication-timing control.The EMBO journal · 2018Article
- Peripheral re-localization of constitutive heterochromatin advances its replication timing and impairs maintenance of silencing marks.Nucleic acids research · 2018Article
- Replicating Large Genomes: Divide and Conquer.Molecular cell · 2016Review
- Mechanism of gene amplification via yeast autonomously replicating sequences.TheScientificWorldJournal · 2015Article
- Chromatin structure and replication origins: determinants of chromosome replication and nuclear organization.Journal of molecular biology · 2014Review
- Protein phosphatase 1 recruitment by Rif1 regulates DNA replication origin firing by counteracting DDK activity.Cell reports · 2014Article
- Location, location, location: it's all in the timing for replication origins.Genes & development · 2013Review
- Structure and function in the budding yeast nucleus.Genetics · 2012Review
- The sub-cellular localization of Sulfolobus DNA replication.Nucleic acids research · 2012Article
- TFIIIC localizes budding yeast ETC sites to the nuclear periphery.Molecular biology of the cell · 2012Article
- Functional centromeres determine the activation time of pericentric origins of DNA replication in Saccharomyces cerevisiae.PLoS genetics · 2012Article
- G2 phase chromatin lacks determinants of replication timing.The Journal of cell biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Peripheral nuclear localization of chromosomal loci correlates with late replication in yeast and metazoan cells. To test whether peripheral positioning can impose late replication, we examined whether artificial tethering of an early-initiating replication origin to the nuclear periphery delays its replication in budding yeast. We tested the effects of three different peripheral tethering constructs on the time of replication of the early replication origin ARS607. Using the dense-isotope transfer method to assess replication time, we found that ARS607 still replicates early when tethered to the nuclear periphery using the Yif1 protein or a fragment of Sir4, whereas tethering using a Yku80 construct produces only a very slight replication delay. Single-cell microscopic analysis revealed no correlation between peripheral positioning of ARS607 in individual cells and delayed replication. Overall, our results demonstrate that a replication origin can initiate replication early in S phase, even if artificially relocated to the nuclear periphery.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.